Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PHLDB2 Primary Antibodies »

Product Specifications

Immunogen

The immungoen recognized by the antibody maps to a region between residue 250 to 300 of human LL5beta using the numbering given in entry NP_001127910.1.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PHLDB2 Antibody - BSA Free

Western Blot: PHLDB2 Antibody [NBP2-36546]

Western Blot: PHLDB2 Antibody [NBP2-36546]

Western Blot: PHLDB2 Antibody [NBP2-36546] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-LL5beta antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes
Immunoprecipitation: PHLDB2 Antibody [NBP2-36546]

Immunoprecipitation: PHLDB2 Antibody [NBP2-36546]

Immunoprecipitation: PHLDB2 Antibody [NBP2-36546] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. For blotting immunoprecipitated LL5beta, it was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for PHLDB2 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg

Western Blot

1:2000 - 1:10000
Application Notes
Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Validation by IP/Western Blot was performed using a 4-8% SDS-PAGE gel.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PHLDB2

Pleckstrin homology-like domain family B member 2 (LL5beata) seems to be involved in the assembly of the postsynaptic apparatus. LL5beta may play a role in acetyl-choline receptor (AChR) aggregation in the postsynaptic membrane (By similarity).

Alternate Names

pleckstrin homology-like domain, family B, member 2

Gene Symbol

PHLDB2

UniProt

NP_001127910.1 (Human)

Additional PHLDB2 Products

Product Documents for PHLDB2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PHLDB2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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