phospho-GABA-B R2 (S892) Antibody

R&D Systems | Catalog # PPS073

R&D Systems
Discontinued Product
PPS073 has been discontinued. View all GABA-B R2 products.

Key Product Details

Species Reactivity

Human, Mouse, Rat, Xenopus

Applications

Western Blot, Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all GABA-B R2 Primary Antibodies »

Product Specifications

Immunogen

Phosphopeptide corresponding to amino acid residues surrounding the phospho-S892 of GABAB R2

Specificity

Human, mouse, rat, and Xenopus ~110 kDa GABAB Receptor, R2 subunit phosphorylated at S892

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for phospho-GABA-B R2 (S892) Antibody

Detection of Phospho-GABABR2 (S892) antibody by Western Blot

Detection of Phospho-GABABR2 (S892) by Western Blot

Western blots of COS-7 cells transiently expressing mutant GABABR2 (S892A) (lane 1), wild type GABABR2 (lane 2) or control untransfected cells (lane 3) were lysed and 50 mg of protein from each sample was separated by SDS-PAGE and transferred to a nitrocellulose membrane. Blots were then immunolabeled with rabbit anti-phospho-GABABR2 (S892) (A). In B, the membrane was pre-treated with lambda -PPase (1200 units for 30 minutes) before being exposed to the anti-GABABReceptor, R2 subunit (S892). The immunolabeling is completely eliminated by treatment with lambda -PPase.

Applications for phospho-GABA-B R2 (S892) Antibody

Application
Recommended Usage

Immunofluorescence

1:100 - 1:250 dilution
Sample: (Couve, et al. 2002)

Western Blot

1:1000 dilution
Sample: COS‑7 African green monkey SV40 transformed kidney fibroblast-like cell line transfected with GABAB R2

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: GABA-B R2

The GABA-B ( gamma -aminobutyric acid-type B) receptors (GABABR) are C family members of the GPCR superfamily. There are only two family members, GABARB1(a & b) and GABARB2, which form a nondisulfide-linked heterodimeric receptor in the native state. GABARB heterodimers are found both pre- and post-synaptically, where they modulate glutamate release presynaptically, and prolong neuronal hyperpolarization postsynaptically. Rat GABARB2 is a 110 kDa, 900 amino acid (aa), 7-transmembrane glycoprotein. It has an extended, 442 aa N-terminal extracellular region that contains the GABA binding site. Unlike most other GPCRs, PKA phosphorylation of the GABAB2 cytoplasmic tail does not promote GABAB2 internalization. To the contrary, phosphorylation of S892 promotes surface expression, while dephosphorylation promotes internalization. Across species (human to frog to mouse to rat), there is 88% aa identity in the 200 aa cytoplasmic tail, and 85% aa identity over the entire mature receptor.

References

  1. Kornau, H-C. (2006) Cell Tissue Res. 326:517.
  2. Huang, Z. (2006) Neuron 50:521.
  3. Fairfax, B.J. et al. (2004) J. Biol. Chem. 279:12565.
  4. White, J.H. et al. (1998) Nature 396:679.
  5. Kaupmann, K. et al. (1998) Nature 396:683.

Long Name

gamma-Aminobutyric Acid Type B Receptor Subunit 2

Alternate Names

GABABR2, GABBR2, GPR51, GPRC3B

Entrez Gene IDs

9568 (Human); 83633 (Rat)

Gene Symbol

GABBR2

Additional GABA-B R2 Products

Product Documents for phospho-GABA-B R2 (S892) Antibody

Certificate of Analysis

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Product Specific Notices for phospho-GABA-B R2 (S892) Antibody

For research use only

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Protocols

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FAQs for phospho-GABA-B R2 (S892) Antibody

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  • Q: Are there neutralizing peptides for this antibody?

    A: There is no neutralizing peptide available for this antibody.

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