Phospho-GABA-B R2 (S892) Antibody

Name: Phospho-GABA-B R2 (S892) Antibody
Brand: R&D Systems
SKU: PPS073

Catalog #: PPS073 Datasheet / COA / SDS

Discontinued Product

PPS073 has been discontinued.

View all GABA-B R2 products.

Detection of Phospho-GABABR2 (S892) by Western Blot

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Phospho-GABA-B R2 (S892) Antibody Summary

Species Reactivity

Human, Mouse, Rat, Xenopus

Specificity

Human, mouse, rat, and Xenopus ~110 kDa GABA_B Receptor, R2 subunit phosphorylated at S892

Source

Polyclonal Rabbit IgG

Purification

Antigen Affinity-purified

Immunogen

Phosphopeptide corresponding to amino acid residues surrounding the phospho-S892 of GABA_B R2

Formulation

100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1:1000 dilution

See below

Immunofluorescence

1:100 - 1:250 dilution

(Couve, et al. 2002)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Phospho-GABA_BR2 (S892) by Western Blot Western blots of COS-7 cells transiently expressing mutant GABA_BR2 (S892A) (lane 1), wild type GABA_BR2 (lane 2) or control untransfected cells (lane 3) were lysed and 50 mg of protein from each sample was separated by SDS-PAGE and transferred to a nitrocellulose membrane. Blots were then immunolabeled with rabbit anti-phospho-GABA_BR2 (S892) (A). In B, the membrane was pre-treated with lambda -PPase (1200 units for 30 minutes) before being exposed to the anti-GABA_BReceptor, R2 subunit (S892). The immunolabeling is completely eliminated by treatment with lambda -PPase.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: GABA-B R2

The GABA-B ( gamma -aminobutyric acid-type B) receptors (GABA_BR) are C family members of the GPCR superfamily. There are only two family members, GABA_{RB1(a & b)} and GABA_RB2, which form a nondisulfide-linked heterodimeric receptor in the native state. GABA_RB heterodimers are found both pre- and post-synaptically, where they modulate glutamate release presynaptically, and prolong neuronal hyperpolarization postsynaptically. Rat GABA_RB2 is a 110 kDa, 900 amino acid (aa), 7-transmembrane glycoprotein. It has an extended, 442 aa N-terminal extracellular region that contains the GABA binding site. Unlike most other GPCRs, PKA phosphorylation of the GABA_B2 cytoplasmic tail does not promote GABA_B2 internalization. To the contrary, phosphorylation of S892 promotes surface expression, while dephosphorylation promotes internalization. Across species (human to frog to mouse to rat), there is 88% aa identity in the 200 aa cytoplasmic tail, and 85% aa identity over the entire mature receptor.

References

Kornau, H-C. (2006) Cell Tissue Res. 326:517.
Huang, Z. (2006) Neuron 50:521.
Fairfax, B.J. et al. (2004) J. Biol. Chem. 279:12565.
White, J.H. et al. (1998) Nature 396:679.
Kaupmann, K. et al. (1998) Nature 396:683.

Long Name

gamma-Aminobutyric Acid Type B Receptor Subunit 2

Entrez Gene IDs

9568 (Human); 83633 (Rat)

Alternate Names

G protein-coupled receptor 51; GABA B R2; GABABR2; GABA-BR2; GABA-B-R2; GABABR2GABA-B receptor 2; GABBR2; gamma-aminobutyric acid (GABA) B receptor, 2; gamma-aminobutyric acid type B receptor subunit 2; gb2; GPR51; GPR51GABA-B receptor, R2 subunit; GPRC3B; GPRC3BFLJ36928; G-protein coupled receptor 51; HG20HRIHFB2099