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Rat PKC beta 1 ELISA Kit (Colorimetric)

Name: Rat PKC beta 1 ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP3-55697

Novus Biologicals | Catalog # NBP3-55697

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Product Documents

Product Specifications
Precision
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Sample Type & Volume Required Per Well

Tissue homogenates, cell lysates and other biological fluids (100 uL)

Sensitivity

0.057 ng/mL (example only; lot dependent)

Assay Range

0.156 - 10 ng/mL (example only; lot dependent)

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Rat

Description

Assay Length: 4.5 hours

精度

Intra-Assay Precision (Precision within an assay) %CV 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV 12 (example only; lot dependent)

Kit Contents for Rat PKC beta 1 ELISA Kit (Colorimetric)

Pre-coated 96T strip plate, Plate sealer for 96 wells, Standard, Diluent Buffer, Detection Reagent A, Detection Reagent B, TMB Substrate, Stop Solution, Wash Buffer (30 x concentrate), Instruction manual

Preparation and Storage

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: PKC beta 1

Protein Kinase C (PKC) beta 1 is a member of the PKC family of serine/threonine protein kinases. It is 671 amino acids (aa) in length with a predicted molecular mass of 77 kDa. Human PKC beta 1 shares 98% aa sequence identity with the mouse and rat orthologs. A second PKC beta splice variant, PKC beta 2, contains an alternate C-terminal sequence. Conventional PKC isoforms, including PKC beta 1, require calcium and phosphatidylserine, diacylglycerol (DAG), or exogenous phorbol esters for activation. PKC beta 1 plays a role in B cell receptor signaling and histone demethylation.

Long Name

Protein Kinase C beta 1

Alternate Names

PRKCB, PRKCB1, PRKCB2

Gene Symbol

PRKCB

Additional PKC beta 1 Products

Product Documents for Rat PKC beta 1 ELISA Kit (Colorimetric)

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Certificate of Analysis

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Product Specific Notices for Rat PKC beta 1 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.