POLR1C Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87114

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (95%), Rat (93%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all POLR1C Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids:NNTSIVQDEILAHRLGLIPIHADPRLFEYRNQGDEEGTEIDTLQFRLQVRCTRNPHAAKDSSDPNELYVNHKVYTRHMTWIPLGNQADLFPEGTIRPVHD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for POLR1C Antibody - BSA Free

Western Blot: POLR1C Antibody [NBP1-87114]

Western Blot: POLR1C Antibody [NBP1-87114]

Western Blot: POLR1C Antibody [NBP1-87114] - Analysis in control (vector only transfected HEK293T lysate) and POLR1C over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: POLR1C Antibody [NBP1-87114]

Immunocytochemistry/ Immunofluorescence: POLR1C Antibody [NBP1-87114]

Immunocytochemistry/Immunofluorescence: POLR1C Antibody [NBP1-87114] - Staining of human cell line A-431 shows positivity in nucleus but not nucleoli. Antibody staining is shown in green.
POLR1C Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: POLR1C Antibody - BSA Free [NBP1-87114]

Chromatin Immunoprecipitation-exo-Seq: POLR1C Antibody - BSA Free [NBP1-87114]

ChIP-Exo-Seq composite graph for Anti-POLR1C (NBP1-87114) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for POLR1C Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Western Blot

0.04-0.4 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: POLR1C

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I and III which synthesize ribosomal RNA precursors and small RNAs, such as 5S rRNA and tRNAs, respectively. RPAC1 is part of the Pol core element with the central large cleft and probably a clamp element that moves to open and close the cleft

Alternate Names

AC40, DNA-directed RNA polymerase I subunit C, DNA-directed RNA polymerases I and III 40 kDa polypeptide, POLR1E, polymerase (RNA) I polypeptide C, 30kDa, RNA polymerase I subunit, RPA39RNA polymerases I and III subunit AC1, RPA40TCS3, RPA5RPAC1DNA-directed RNA polymerases I and III subunit RPAC1, RPC40

Gene Symbol

POLR1C

Additional POLR1C Products

Product Documents for POLR1C Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for POLR1C Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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