Porcine IL-8/CXCL8 Quantikine ELISA Kit

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P8000
Porcine CXCL8/IL-8 ELISA Standard Curve
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Citations (22)
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Porcine IL-8/CXCL8 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (100 uL)
Sensitivity
6.7 pg/mL
Assay Range
62.5 - 4,000 pg/mL (Cell Culture Supernates, Serum)
Specificity
Natural and recombinant porcine IL-8
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Porcine IL-8 Immunoassay is a 4.5 hour solid phase ELISA designed to measure porcine IL-8 in cell culture supernates and serum. It utilizes E. coli-expressed recombinant porcine IL-8 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately measure or quantify the recombinant porcine IL-8. Results obtained using natural porcine IL-8 show dose response curves that are parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural porcine IL-8.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 97 748 1607 106 717 1641
Standard Deviation 9.4 64 106 9 49 108
CV% 9.7 8.6 6.6 8.5 6.8 6.6

Recovery

The recovery of porcine IL-8 spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=7) 108 80-120
Serum (n=7) 91 80-100

Linearity

To assess the linearity of the assay, five or more samples containing and/or spiked with various concentrations of porcine IL-8 in each matrix were diluted with Calibrator Diluent and then assayed.
Porcine CXCL8/IL-8 ELISA Linearity

Data Examples

Porcine CXCL8/IL-8 ELISA Standard Curve

Product Datasheets

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-8/CXCL8

Interleukin-8 (IL-8), also known as CXCL8, is a chemokine that is upregulated at sites of inflammation where it promotes neutrophil infiltration and activation. IL-8 can form homodimers and heterodimers with CXCL4/PF4. Its bioactivity is regulated by proteolytic truncations, citrullination, and the decoy receptor DARC. IL-8 signals through CXCR1/IL-8 RA, which is also used by CXCL6, and through CXCR2/IL-8 RB, which is used by multiple CXC chemokines. IL-8 also binds to Serpin A1/alpha-1 Antitrypsin which prevents IL-8 interaction with CXCR1.

Long Name:
Interleukin 8
Entrez Gene IDs:
3576 (Human); 396880 (Porcine); 403850 (Canine); 493836 (Feline)
Alternate Names:
3-10C; AMCF-I; C-X-C motif chemokine 8; CXCL8; CXCL8SCYB8; Emoctakin; GCP1; GCP-1TSG-1; IL8; IL-8; interleukin 8; K60; LAI; LECT; LUCT; LYNAP; MDNCF; MDNCFb-ENAP; member 8; MONAP; MONAPGCP1; NAF; NAP1; NAP-1NAP1; NCF; Neutrophil-activating protein 1; Protein 3-10C; T cell chemotactic factor; T-cell chemotactic factor; TCF; TSG1
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Assay Diluent
  4.   Add 50 µL of Assay Diluent to each well.

  5. 100 µL Standard, Control, or Sample
  6.   Add 100 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  10.   Aspirate and wash 5 times.

  11. 120 µL Substrate Solution
  12.   Add 120 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

  13. 120 µL Stop Solution
  14.   Add 120 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Porcine IL-8/CXCL8 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

22 Citations: Showing 1 - 10
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  1. Efficacy of Mycotoxin Detoxifiers on Health and Growth of Newly-Weaned Pigs under Chronic Dietary Challenge of Deoxynivalenol
    Authors: DM Holanda, SW Kim
    Toxins (Basel), 2020;12(5):.
    Species: Porcine
    Sample Types: Tissue Homogenates
  2. Immuno-modulating properties of Tulathromycin in porcine monocyte-derived macrophages infected with porcine reproductive and respiratory syndrome virus
    Authors: D Desmonts d, R Moges, A Siddiq, T Allain, TD Feener, GP Muench, N McKenna, RM Yates, AG Buret
    PLoS ONE, 2019;14(8):e0221560.
    Species: Porcine
    Sample Types: Cell Culture Supernates
  3. Sepsis causes right ventricular myocardial inflammation independent of pulmonary hypertension in a porcine sepsis model
    Authors: SE Pischke, S Hestenes, HT Johansen, H Fure, JF Bugge, A Espinoza, H Skulstad, T Edvardsen, E Fosse, TE Mollnes, PS Halvorsen, EW Nielsen
    PLoS ONE, 2019;14(6):e0218624.
    Species: Porcine
    Sample Types: Plasma
  4. Effects of divergent selection upon adrenocortical activity on immune traits in pig
    Authors: J Hervé, E Terenina, K Haurogné, E Bacou, E Kulikova, M Allard, Y Billon, JM Bach, P Mormède, B Lieubeau
    BMC Vet. Res., 2019;15(1):71.
    Species: Porcine
    Sample Types: Cell Culture Supernates
  5. Inactivating hepatitis C virus in donor lungs using light therapies during normothermic ex vivo lung perfusion
    Authors: M Galasso, JJ Feld, Y Watanabe, M Pipkin, C Summers, A Ali, R Qaqish, M Chen, RVP Ribeiro, K Ramadan, L Pires, VS Bagnato, C Kurachi, V Cherepanov, G Moonen, A Gazzalle, TK Waddell, M Liu, S Keshavjee, BC Wilson, A Humar, M Cypel
    Nat Commun, 2019;10(1):481.
    Species: Porcine
    Sample Types: Tissue Homogenates
  6. Pulmonary Effects of Remote Ischemic Preconditioning in a Porcine Model of Ventilation-induced Lung Injury
    Authors: A Bergmann, T Schilling, G Hedenstier, K Ahlgren, A Larsson, M Kretzschma, A Kozian, T Hachenberg
    Respir Physiol Neurobiol, 2018;0(0):.
    Species: Porcine
    Sample Types: Tissue Homogenates
  7. Butyrate modifies intestinal barrier function in IPEC-J2 cells through a selective upregulation of tight junction proteins and activation of the Akt signaling pathway
    Authors: H Yan, KM Ajuwon
    PLoS ONE, 2017;12(6):e0179586.
    Species: Porcine
    Sample Types: Cell Culture Supernates
  8. Effect of Lactobacillus salivarius bacteriocin Abp118 on the mouse and pig intestinal microbiota.
    Authors: Riboulet-Bisson E, Sturme MH, Jeffery IB
    PLoS ONE, 2012;7(2):e31113.
    Species: Porcine
    Sample Types: Serum
  9. Epithelial proinflammatory response and curcumin-mediated protection from staphylococcal toxic shock syndrome toxin-1.
    Authors: Schaefers MM, Breshears LM, Anderson MJ, Lin YC, Grill AE, Panyam J, Southern PJ, Schlievert PM, Peterson ML
    PLoS ONE, 2012;7(3):e32813.
    Species: Porcine
    Sample Types: Cell Culture Supernates
  10. Efficacy of linezolid compared to vancomycin in an experimental model of pneumonia induced by methicillin-resistant Staphylococcus aureus in ventilated pigs.
    Authors: Martinez-Olondris P, Rigol M, Soy D, Guerrero L, Agusti C, Quera MA, Li Bassi G, Esperatti M, Luque N, Liapikou M, Filella X, Marco F, de la Bellacasa JP, Torres A
    Crit. Care Med., 2012;40(1):162-8.
    Species: Porcine
    Sample Types: Serum
  11. Ornithodoros moubata complement inhibitor is an equally effective C5 inhibitor in pigs and humans.
    Authors: Barratt-Due A, Thorgersen EB, Lindstad JK, Pharo A, Lissina O, Lambris JD, Nunn MA, Mollnes TE
    J. Immunol., 2011;187(9):4913-9.
    Species: Porcine
    Sample Types: Plasma
  12. Plasma concentrations of inflammatory cytokines rise rapidly during ECMO-related SIRS due to the release of preformed stores in the intestine.
    Authors: McILwain RB, Timpa JG, Kurundkar AR
    Lab. Invest., 2010;90(1):128-39.
    Species: Porcine
    Sample Types: Plasma
  13. CD14 inhibition efficiently attenuates early inflammatory and hemostatic responses in Escherichia coli sepsis in pigs.
    Authors: Thorgersen EB, Hellerud BC, Nielsen EW, Barratt-Due A, Fure H, Lindstad JK, Pharo A, Fosse E, Tonnessen TI, Johansen HT, Castellheim A, Mollnes TE
    FASEB J., 2010;24(3):712-22.
    Species: Porcine
    Sample Types: Plasma
  14. Dissecting the effects of lipopolysaccharides from nonlipopolysaccharide molecules in experimental porcine meningococcal sepsis.
    Authors: Hellerud BC, Nielsen EW, Thorgersen EB, Lindstad JK, Pharo A, Tonnessen TI, Castellheim A, Mollnes TE, Brandtzaeg P
    Crit. Care Med., 2010;38(6):1467-74.
    Species: Porcine
    Sample Types: Plasma
  15. Metabolic activity of the enteric microbiota influences the fatty acid composition of murine and porcine liver and adipose tissues.
    Authors: Wall R, Ross RP, Shanahan F, O'Mahony L, O'Mahony C, Coakley M, Hart O, Lawlor P, Quigley EM, Kiely B, Fitzgerald GF, Stanton C
    Am. J. Clin. Nutr., 2009;89(5):1393-401.
    Species: Porcine
    Sample Types: Cell Culture Supernates
  16. Improved pulmonary function by acid sphingomyelinase inhibition in a newborn piglet lavage model.
    Authors: von Bismarck P, Wistadt CF, Klemm K, Winoto-Morbach S, Uhlig U, Schutze S, Adam D, Lachmann B, Uhlig S, Krause MF
    Am. J. Respir. Crit. Care Med., 2008;177(11):1233-41.
    Species: Porcine
    Sample Types: BALF
  17. Effects of glucocorticoids in ventilated piglets with severe pneumonia.
    Authors: Sibila O, Luna CM, Agusti C, Baquero S, Gando S, Patron JR, Morato JG, Absi R, Bassi N, Torres A
    Eur. Respir. J., 2008;32(4):1037-46.
    Species: Porcine
    Sample Types: Serum
  18. Cytokine secretion depends on Galalpha(1,3)Gal expression in a pig-to-human whole blood model.
    Authors: Saethre M, Schneider MK, Lambris JD, Magotti P, Haraldsen G, Seebach JD, Mollnes TE
    J. Immunol., 2008;180(9):6346-53.
    Species: Porcine
    Sample Types: Cell Culture Supernates
  19. Experimental Pseudomonas aeruginosa pneumonia: evaluation of the associated inflammatory response.
    Authors: Sibila O, Agusti C, Torres A, Baquero S, Gando S, Patron JR, Morato JG, Goffredo DH, Bassi N, Luna CM
    Eur. Respir. J., 2007;30(6):1167-72.
    Species: Porcine
    Sample Types: Serum
  20. Dietary fatty acid composition rather than vitamin E supplementation influence ex vivo cytokine and eicosanoid response of porcine alveolar macrophages.
    Authors: Moller S, Lauridsen C
    Cytokine, 2006;35(1):6-12.
    Species: Porcine
    Sample Types: Cell Culture Supernates
  21. Fibrinogen induces chemotactic activity in endothelial cells.
    Authors: Seeger FH, Blessing E, Gu L, Bornhold R, Denger S, Kreuzer J
    Acta Physiol. Scand., 2002;176(2):109-15.
    Species: Porcine
    Sample Types: Cell Culture Supernates
  22. Ex vivo adenoviral vector gene delivery results in decreased vector-associated inflammation pre- and post-lung transplantation in the pig.
    Authors: Yeung JC, Wagnetz D, Cypel M, Rubacha M, Koike T, Chun YM, Hu J, Waddell TK, Hwang DM, Liu M, Keshavjee S
    Mol. Ther., 212;20(6):1204-11.
    Species: Porcine
    Sample Types: Plasma

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