RASGRP3 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # H00025780-D01P

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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all RASGRP3 Primary Antibodies »

Product Specifications

Immunogen

RASGRP3 (NP_733772.1, 1 a.a. - 690 a.a.) full-length human protein. MGSSGLGKAATLDELLCTCIEMFDDNGELDNSYLPRIVLLMHRWYLSSTELAEKLLCMYRNATGESCNEFRLKICYFMRYWILKFPAEFNLDLGLIRMTEEFREVASQLGYEKHVSLIDISSIPSYDWMRRVTQRKKVSKKGKACLLFDHLEPIELAEHLTFLEHKSFRRISFTDYQSYVIHGCLENNPTLERSIALFNGISKWVQLMVLSKPTPQQRAEVITKFINVAKKLLQLKNFNTLMAVVGGLSHSSISRLKETHSHLSSEVTKNWNEMTELVSSNGNYCNYRKAFADCDGFKIPILGVHLKDLIAVHVIFPDWTEENKVNIVKMHQLSVTLSELVSLQNASHHLEPNMDLINLLTLSLDLYHTEDDIYKLSLVLEPRNSKSQPTSPTTPNKPVVPLEWALGVMPKPDPTVINKHIRKLVESVFRNYDHDHDGYISQEDFESIAANFPFLDSFCVLDKDQDGLISKDEMMAYFLRAKSQLHCKMGPGFIHNFQEMTYLKPTFCEHCAGFLWGIIKQGYKCKDCGANCHKQCKDLLVLACRRFARAPSLSSGHGSLPGSPSLPPAQDEVFEFPGVTAGHRDLDSRAITLVTGSSRKISVRLQRATTSQATQTEPVWSEAGWGDSGSHTFPKMKSKFHDKAAKDKGFAKWENEKPRVHAGVDVVDRGTEFELDQDEGEETRQDGEDG

Specificity

RAS guanyl releasing protein 3 (calcium and DAG-regulated)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RASGRP3 Antibody - Azide and BSA Free

Western Blot: RASGRP3 Antibody [H00025780-D01P]

Western Blot: RASGRP3 Antibody [H00025780-D01P]

Western Blot: RASGRP3 Antibody [H00025780-D01P] - Analysis of RASGRP3 expression in mouse kidney.
Immunocytochemistry/ Immunofluorescence: RASGRP3 Antibody [H00025780-D01P]

Immunocytochemistry/ Immunofluorescence: RASGRP3 Antibody [H00025780-D01P]

Immunocytochemistry/Immunofluorescence: RASGRP3 Antibody [H00025780-D01P] - Analysis of antibody to RASGRP3 on HeLa cell. Antibody concentration 30 ug/ml.
Western Blot: RASGRP3 Antibody [H00025780-D01P]

Western Blot: RASGRP3 Antibody [H00025780-D01P]

Western Blot: RASGRP3 Antibody [H00025780-D01P] - Analysis of RASGRP3 expression in transfected 293T cell line by RASGRP3 polyclonal antibody.Lane 1: RASGRP3 transfected lysate(78.30 KDa).Lane 2: Non-transfected lysate.

Applications for RASGRP3 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is reactive against mammalian transfected lysate in western blot. May also be used for ELISA.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RASGRP3

Members of the RAS (see HRAS; MIM 190020) subfamily of GTPases function in signal transduction as GTP/GDP-regulated switches that cycle between inactive GDP- and active GTP-bound states. Guanine nucleotide exchange factors (GEFs), such as RASGRP3, serve as RAS activators by promoting acquisition of GTP to maintain the active GTP-bound state and are the key link between cell surface receptors and RAS activation (Rebhun et al., 2000 [PubMed 10934204]).[supplied by OMIM]

Alternate Names

Calcium and DAG-regulated guanine nucleotide exchange factor III, GRP3KIAA0846ras guanyl-releasing protein 3, Guanine nucleotide exchange factor for Rap1, RAS guanyl releasing protein 3 (calcium and DAG-regulated)

Entrez Gene IDs

25780 (Human)

Gene Symbol

RASGRP3

UniProt

NP_733772.1 (Human)

Additional RASGRP3 Products

Product Documents for RASGRP3 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for RASGRP3 Antibody - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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