Rat IL-10 Quantikine ELISA Kit

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R1000
PR1000
SR1000
Rat IL-10 ELISA Cell Culture Supernate Standard Curve
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Product Details
Procedure
Citations (38)
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Rat IL-10 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL)
Sensitivity
10 pg/mL
Assay Range
31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma)
Specificity
Natural and recombinant rat IL-10
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Rat IL-10 Immunoassay is a 4.5 hour solid phase ELISA designed to measure rat IL-10 levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant rat IL-10 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant rat IL-10 accurately. Results obtained using natural rat IL-10 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural rat IL-10.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Serum, EDTA Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 138 270 660 132 270 682
Standard Deviation 6.3 9 16.1 13.1 19.7 59.8
CV% 4.6 3.3 2.4 9.9 7.3 8.8

Cell Culture Supernates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 103 218 582 114 236 598
Standard Deviation 5.7 9.5 17.2 10.8 16.4 42.6
CV% 5.5 4.4 3 9.5 6.9 7.1

Recovery

The recovery of rat IL-10 spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=5) 106 96-118
EDTA Plasma (n=6) 100 88-115
Serum (n=6) 94 82-112

Linearity

To assess the linearity of the assay, five or more samples spiked with various concentrations of rat IL-10 in each matrix were diluted with the appropriate Calibrator Diluent and then assayed.
Rat IL-10 ELISA Linearity

Data Examples

Rat IL-10 ELISA Cell Culture Supernate Standard Curve

Rat IL-10 ELISA Serum/Plasma Standard Curve

Product Datasheets

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-10

Interleukin-10 (IL-10), also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 alpha -helical cytokine family that also includes IL-19, IL-20, IL-22, IL-24, and IL-26/AK155 (1-3). IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts. Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells (4, 5). Mature rat IL-10 shares 85% amino acid sequence identity with mouse IL-10 and 71% - 79% with bovine, canine, equine, feline, guinea pig, human, ovine, and porcine IL-10. It contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently-associated homodimer (4, 6, 7). 

IL-10 mediates its biological activities through a heteromeric receptor complex composed of the type II cytokine receptor subunits IL-10 R alpha and IL-10 R beta. IL-10 R alpha is a 110 kDa transmembrane glycoprotein that is expressed on lymphocytes, NK cells, macrophages, monocytes, astrocytes, intestinal epithelial cells, cytotrophoblasts, and activated hepatic stellate cells (8-13), while the 75 kDa transmembrane IL-10 R beta is widely expressed (14, 15). The IL-10 dimer binds to two IL-10 R alpha chains, triggering recruitment of two IL-10 R beta chains (14, 15). IL-10 R beta does not bind IL-10 directly but is required for signal transduction. IL-10 R beta also associates with IL-20 R alpha, IL-22 R alpha 1, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL-29 (16-18). 
The involvement of IL-10 in immunoregulation includes both suppressive and stimulatory effects. It functions as an anti-inflammatory cytokine by inhibiting the expansion and activation of Th1 cells and Th17 cells (19-21), and by promoting the development of M2 macrophages (21). Its expression by immunosuppressive regulatory T cells (Treg) and regulatory B cells is important for Treg proliferation (19). Within a tumor microenvironment, however, IL-10 inhibits the expansion of Treg as well as myeloid-derived suppressor cells (22, 23). IL-10 induces the intratumoral accumulation and activation of CD8+ T cells (24, 25). IL-10 exerts protective effects including limiting tissue damage in arthritic inflammation (19) and promoting muscle regeneration after injury (21), but it also contributes to the persistence of viral infections (26). The levels of IL-10 have been found to be elevated in Sjogren's syndrome (saliva), primary CNS lymphoma (cerebrospinal fluid), and ovarian cancer (serum and ascites) (27-29). Its levels have been found to be decreased in the serum of patients with recurrent heart attacks or during preeclampsia and also in the seminal fluid of infertile men (30-32).

Long Name:
Interleukin 10
Entrez Gene IDs:
3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 493683 (Feline); 100135553 (Guinea Pig); 2949786 (Viral)
Alternate Names:
CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; GVHDS; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Assay Diluent
  4.   Add 50 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

  8. 100 µL Conjugate
  9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  10.   Aspirate and wash 5 times.

  11. 100 µL Substrate Solution
  12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 100 µL Stop Solution
  14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Rat IL-10 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

38 Citations: Showing 1 - 10
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  1. Oxidative Stress Parameters in the Liver of Growing Male Rats Receiving Various Alcoholic Beverages
    Authors: A Ko?ota, D G??bska, M Oczkowski, J Gromadzka-
    Nutrients, 2020;12(1):.
    Species: Rat
    Sample Types: Tissue Homogenates
  2. Neutrophil activation causes tumor regression in Walker 256 tumor-bearing rats
    Authors: WMT Kuwabara, J Andrade-Si, JNB Pereira, JH Scialfa, J Cipolla-Ne
    Sci Rep, 2019;9(1):16524.
    Species: Rat
    Sample Types: BALF
  3. Low-Energy Extracorporeal Shock Wave Ameliorates Streptozotocin Induced Diabetes and Promotes Pancreatic Beta Cells Regeneration in a Rat Model
    Authors: CC Hsiao, CC Lin, YS Hou, JY Ko, CJ Wang
    Int J Mol Sci, 2019;20(19):.
    Species: Rat
    Sample Types: Tissue Lysates
  4. Human mesenchymal stromal cells ameliorate complement induced inflammatory cascade and improve renal functions in a rat model of ischemia-reperfusion induced acute kidney injury
    Authors: S Zilberman-, R Abu-Hamad, R Zarura, M Sova, Y Hachmo, M Stark, S Neuman, S Slavin, S Efrati
    PLoS ONE, 2019;14(9):e0222354.
    Species: Rat
    Sample Types: Serum
  5. Low-Energy Extracorporeal Shock Wave Therapy Ameliorates Kidney Function in Diabetic Nephropathy
    Authors: CC Hsiao, WH Huang, KH Cheng, CT Lee
    Oxid Med Cell Longev, 2019;2019(0):8259645.
    Species: Rat
    Sample Types: Tissue Lysate
  6. Autophagy mediates tumor necrosis factor-?-induced phenotype switching in vascular smooth muscle A7r5 cell line
    Authors: M García-Mig, JA Riquelme, I Norambuena, PE Morales, F Sanhueza-O, C Nuñez-Soto, D Mondaca-Ru, N Cancino-Ar, A San Martín, M Chiong
    PLoS ONE, 2018;13(5):e0197210.
    Species: Rat
    Sample Types: Cell Culture Supernates
  7. Toll-like receptor 3 activation impairs excitability and synaptic activity via TRIF signalling in immature rat and human neurons
    Authors: L Ritchie, R Tate, LH Chamberlai, G Robertson, M Zagnoni, T Sposito, S Wray, JA Wright, CE Bryant, NJ Gay, TJ Bushell
    Neuropharmacology, 2018;135(0):1-10.
    Species: Rat
    Sample Types: Cell Culture Supernates
  8. The local cytokine and growth factor response to rhBMP-2 after spinal fusion
    Authors: JD Koerner, DZ Markova, GD Schroeder, BP Calio, A Shah, CW Brooks, AR Vaccaro, DG Anderson, CK Kepler
    Spine J, 2018;0(0):.
    Species: Rat
    Sample Types: Tissue Lysates
    Applications: ELISA (Standard)
  9. Placenta?derived mesenchymal stem cells improve airway hyperresponsiveness and inflammation in asthmatic rats by modulating the Th17/Treg balance
    Authors: Y Li, H Li, Y Cao, F Wu, W Ma, Y Wang, S Sun
    Mol Med Rep, 2017;0(0):.
  10. A Novel Role of SIRT1/ FGF-21 in Taurine Protection Against Cafeteria Diet-Induced Steatohepatitis in Rats
    Authors: AH Abd Elwaha, BK Ramadan, MF Schaalan, AM Tolba
    Cell. Physiol. Biochem., 2017;43(2):644-659.
    Species: Rat
    Sample Types: Tissue Homogenates
  11. Biological effects of bone marrow mesenchymal stem cells on hepatitis B virus in�vitro
    Authors: WP Zheng, BY Zhang, ZY Shen, ML Yin, Y Cao, HL Song
    Mol Med Rep, 2017;15(5):2551-2559.
    Species: Rat
    Sample Types: Cell Culture Supernates
  12. Investigation of the effect of phlomisoside F on complete Freund's adjuvant-induced arthritis
    Authors: X Zhang, Y Dong, H Dong, W Zhang, F Li
    Exp Ther Med, 2017;13(2):710-716.
    Species: Rat
    Sample Types: Serum
  13. Short-term treatment with taurolidine is associated with liver injury
    Authors: R Fahrner, A Möller, AT Press, A Kortgen, M Kiehntopf, F Rauchfuss, U Settmacher, AS Mosig
    BMC Pharmacol Toxicol, 2017;18(1):61.
    Species: Rat
    Sample Types: Serum
  14. Gene expression profiling and inhibition of adipose tissue accumulation of G. bimaculatus extract in rats on high fat diet.
    Authors: Ahn M, Kim M, Kwon R, Hwang J, Park K
    Lipids Health Dis, 2015;14(0):116.
    Species: Rat
    Sample Types: Serum
  15. Non-invasive technology that improves cardiac function after experimental myocardial infarction: Whole Body Periodic Acceleration (pGz).
    Authors: Uryash A, Bassuk J, Kurlansky P, Altamirano F, Lopez J, Adams J
    PLoS ONE, 2015;10(3):e0121069.
    Species: Rat
    Sample Types: Tissue Homogenates
  16. Preventive effects of probiotic bacteria Lactobacillus plantarum and dietary fiber in chemically-induced mammary carcinogenesis.
    Authors: Kassayova M, Bobrov N, Strojny L, Kiskova T, Mikes J, Demeckova V, Orendas P, Bojkova B, Pec M, Kubatka P, Bomba A
    Anticancer Res, 2014;34(9):4969-75.
    Species: Rat
    Sample Types: Serum
  17. S100A1 is released from ischemic cardiomyocytes and signals myocardial damage via Toll-like receptor 4.
    Authors: Rohde D, Schon C, Boerries M, Didrihsone I, Ritterhoff J, Kubatzky K, Volkers M, Herzog N, Mahler M, Tsoporis J, Parker T, Linke B, Giannitsis E, Gao E, Peppel K, Katus H, Most P
    EMBO Mol Med, 2014;6(6):778-94.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  18. Improvement of spinal non-viral IL-10 gene delivery by D-mannose as a transgene adjuvant to control chronic neuropathic pain.
    Authors: Dengler E, Alberti L, Bowman B, Kerwin A, Wilkerson J, Moezzi D, Limanovich E, Wallace J, Milligan E
    J Neuroinflammation, 2014;11(0):92.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  19. Atrial fibrosis in a chronic murine model of obstructive sleep apnea: mechanisms and prevention by mesenchymal stem cells.
    Authors: Ramos P, Rubies C, Torres M, Batlle M, Farre R, Brugada J, Montserrat J, Almendros I, Mont L
    Respir Res, 2014;15(0):54.
    Species: Rat
    Sample Types: Plasma
  20. Rhamnogalacturonan from Acmella oleracea (L.) R.K. Jansen: gastroprotective and ulcer healing properties in rats.
    Authors: Maria-Ferreira D, da Silva L, Mendes D, Cabrini D, Nascimento A, Iacomini M, Cipriani T, Santos A, Werner M, Baggio C
    PLoS ONE, 2014;9(1):e84762.
    Species: Rat
    Sample Types: Tissue Culture Supernates
  21. Blocking KV1.3 channels inhibits Th2 lymphocyte function and treats a rat model of asthma.
    Authors: Koshy S, Huq R, Tanner M, Atik M, Porter P, Khan F, Pennington M, Hanania N, Corry D, Beeton C
    J Biol Chem, 2014;289(18):12623-32.
    Species: Rat
    Sample Types: BALF
  22. Early-life experience decreases drug-induced reinstatement of morphine CPP in adulthood via microglial-specific epigenetic programming of anti-inflammatory IL-10 expression.
    Authors: Schwarz JM, Hutchinson MR, Bilbo SD
    J. Neurosci., 2011;31(49):17835-47.
    Species: Rat
    Sample Types: Serum
  23. Curcumin protects intestinal mucosal barrier function of rat enteritis via activation of MKP-1 and attenuation of p38 and NF-kappaB activation.
    Authors: Song WB, Wang YY, Meng FS, Zhang QH, Zeng JY, Xiao LP, Yu XP, Peng DD, Su L, Xiao B, Zhang ZS
    PLoS ONE, 2010;5(9):e12969.
    Species: Rat
    Sample Types: Plasma
  24. Vipera aspis venom reduces lethality and down-regulates tumor necrosis factor-alpha in a rat model of LPS-induced sepsis.
    Authors: Frolkis I, Klein Y, Locker C, Adi N, Dahan E, Uretzsky G, Shapira I, Sorkine P
    Cytokine, 2010;49(3):319-24.
    Species: Rat
    Sample Types: Serum
  25. Oridonin promotes CD4+/CD25+ Treg differentiation, modulates Th1/Th2 balance and induces HO-1 in rat splenic lymphocytes.
    Authors: Hu AP, Du JM, Li JY, Liu JW
    Inflamm. Res., 2008;57(4):163-70.
    Species: Rat
    Sample Types: Cell Culture Supernates
  26. Ocular regulatory T cells distinguish monophasic from recurrent autoimmune uveitis.
    Authors: Ke Y, Jiang G, Sun D, Kaplan HJ, Shao H
    Invest. Ophthalmol. Vis. Sci., 2008;49(9):3999-4007.
    Species: Rat
    Sample Types: Aqueous Humor
  27. Cellular recruitment and cytokine generation in a rat model of allergic lung inflammation are differentially modulated by progesterone and estradiol.
    Authors: de Oliveira AP, Domingos HV, Cavriani G, Damazo AS, Dos Santos Franco AL, Oliani SM, Oliveira-Filho RM, Vargaftig BB, de Lima WT
    Am. J. Physiol., Cell Physiol., 2007;293(3):C1120-8.
    Species: Rat
    Sample Types: BALF
  28. Expression of the mu opioid receptor in the human immunodeficiency virus type 1 transgenic rat model.
    Authors: Chang SL, Beltran JA, Swarup S
    J. Virol., 2007;81(16):8406-11.
    Species: Rat
    Sample Types: Serum
  29. Resuscitation from experimental heatstroke by estrogen therapy.
    Authors: Chen SH, Chang FM, Niu KC, Lin MY, Lin MT
    Crit. Care Med., 2006;34(4):1113-8.
    Species: Rat
    Sample Types: Serum
  30. Effects of gabexate mesilate on serum inflammatory cytokines in rats with acute necrotizing pancreatitis.
    Authors: Chen CC, Wang SS, Tsay SH, Lee FY, Lu RH, Chang FY, Lee SD
    Cytokine, 2006;33(2):95-9.
    Species: Rat
    Sample Types: Serum
  31. Biomarker and drug-target discovery using proteomics in a new rat model of sepsis-induced acute renal failure.
    Authors: Holly MK, Dear JW, Hu X, Schechter AN, Gladwin MT, Hewitt SM, Yuen PS, Star RA
    Kidney Int., 2006;70(3):496-506.
    Species: Rat
    Sample Types: Serum
  32. Suppression of autoimmune retinal disease by lovastatin does not require Th2 cytokine induction.
    Authors: Gegg ME, Harry R, Hankey D, Zambarakji H, Pryce G, Baker D, Adamson P, Calder V, Greenwood J
    J. Immunol., 2005;174(4):2327-35.
    Species: Rat
    Sample Types: Cell Culture Supernates
  33. Effects of pyrrolidine dithiocarbamate, an NF-kappaB inhibitor, on cytokine expression and ocular inflammation in experimental autoimmune anterior uveitis.
    Authors: Fang IM, Yang CH, Lin CP, Yang CM, Chen MS
    J Ocul Pharmacol Ther, 2005;21(2):95-106.
    Species: Rat
    Sample Types: Aqueous Humor
  34. Interleukin-10 aerosol reduces proinflammatory mediators in bronchoalveolar fluid of endotoxemic rat.
    Authors: Hofstetter C, Flondor M, Hoegl S, Muhl H, Zwissler B
    Crit. Care Med., 2005;33(10):2317-22.
    Species: Rat
    Sample Types: BALF
  35. Immunotherapy of murine prostate cancer using whole tumour cells killed ex vivo by cytosine deaminase/5-fluorocytosine suicide-gene therapy.
    Authors: Pandha HS, Cook D, Greenhalgh R, Dalgleish A
    BJU Int., 2005;95(9):1336-43.
    Species: Rat
    Sample Types: Cell Culture Supernates
  36. Ameliorating effects of the immunomodulator 3-(2-ethylphenyl)-5-(3-methoxyphenyl)-1H-1,2,4-triazole in an experimental model of colitis in the rat.
    Authors: Stasi MA, Ruggiero V, Ursillo A, Taurelli R, Agliano M, Weber E, Lorenzoni P, Sorrentino V, Pacifici L, Carminati P
    Eur. J. Pharmacol., 2004;494(2):263-72.
    Species: Rat
    Sample Types: Tissue Homogenates
  37. Insulin attenuates the systemic inflammatory response in endotoxemic rats.
    Authors: Jeschke MG, Klein D, Bolder U, Einspanier R
    Endocrinology, 2004;145(9):4084-93.
    Species: Rat
    Sample Types: Tissue Homogenates
  38. Insulin treatment improves hepatic morphology and function through modulation of hepatic signals after severe trauma.
    Authors: Schubert T, Horch RE
    Ann. Surg., 2004;240(2):340-9.
    Species: Rat
    Sample Types: Tissue Homogenates

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Rat IL-10 Quantikine ELISA Kit
By Anonymous on 12/09/2020
Application: Sample Tested: Spinal cord tissue

Excellent repeatable results.


Rat IL-10 Quantikine ELISA Kit
By Anonymous on 03/13/2020
Application: Sample Tested: simulated body fluid

We tested IL-10 levels in a release assay performed in simulated body fluid. The standard curve was good and the CV values were good. We have used kits from other companies and found RnD systems kits to be superior to all other companies. The kits from RnD systems result in much lower background levels and much lower CV levels. The image shows the standard curve and the green outlines the low CV values.