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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
RCAN1 synthetic peptide (coupled to carrier protein) corresponds to amino acids from the central region of human RCAN1S (isoform 2). This sequence is highly conserved in rat and mouse RCAN1, and this sequence is found in all four isoforms of RCAN1. In addition, this sequence has significant homology to RCAN2 and RCAN3. [UniProt# P53805]
Specificity
The antibody detects several isoforms of RCAN1 on SDS-PAGE immunoblots of mouse C2C12, rat PC12, and human Jurkat cells. The predominate isoform detected is the 38 kDa RCAN1L, but the antibody may also detect RCAN2 and RCAN3.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Stable for at least 1 year at -20°C.
Scientific Data Images for RCAN1 Antibody
Western Blot: RCAN1 Antibody [NBP3-23360] -
Western Blot: RCAN1 Antibody [NBP3-23360] - Western blot analysis of RCAN1 expression in human Jurkat (lanes 1 & 4), mouse C2C12 (lanes 2 & 5), and rat PC12 (lanes 3 & 6). The blot was probed with rabbit polyclonal anti-RCAN1 (C-terminus) at 1:1000 and anti-RCAN1 (a.a. 132-140) at 1:500.
Immunocytochemistry/Immunofluorescence: RCAN1 Antibody [NBP3-23360] -
Immunocytochemistry/Immunofluorescence: RCAN1 Antibody [NBP3-23360] - Immunocytochemical labeling of RCAN1 in aldehyde-fixed NGF-differentiated PC12 cells. The cells were labeled with rabbit polyclonal anti-RCAN1 (C-terminus) (NBP3-23371) and anti-RCAN1 (a.a. 132-140) (NBP3-23360) antibodies (Left side). These antibodies were also used in the presence (Right side) of blocking peptide. The antibodies were detected using appropriate secondary antibody conjugated to DyLight(R) 594.Applications for RCAN1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
6:00
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: RCAN1
Alternate Names
Adapt78, calcipressin-1, calcium and oxidant-inducible mRNA, Down syndrome candidate region 1, Down syndrome critical region gene 1, Down syndrome critical region protein 1, DSC1, modulatory calcineurin-interacting protein 1, Myocyte-enriched calcineurin-interacting protein 1, near DSCR proline-rich protein, regulator of calcineurin 1CSP1DSCR1MCIP1RCN1
Gene Symbol
RCAN1
Additional RCAN1 Products
Product Documents for RCAN1 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for RCAN1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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