Recombinant Human CD117/c-kit Protein, CF

Catalog # Availability Size / Price Qty
332-SR-050/CF

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Recombinant Human CD117/c-kit Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit SCF-dependent proliferation of TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 2-6 µg/mL in the presence of 8 ng/mL of Recombinant Human SCF.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human CD117/c-kit protein
Gln26-Thr520
Accession #
N-terminal Sequence
Analysis
Gln26
Predicted Molecular Mass
55.7 kDa
SDS-PAGE
59-63 kDa, reducing conditions

Product Datasheets

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332-SR/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

332-SR/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: CD117/c-kit

Stem Cell Factor Receptor (SCF R), also known as c-Kit and CD117, is a widely expressed 145 kDa receptor tyrosine kinase. It is the cellular homolog of the feline sarcoma virus protein, v-Kit. Binding of SCF R to SCF, also known as Steel Factor and Kit Ligand, promotes the survival, differentiation, and mobilization of progenitor cells in multiple lineages (1-4). Mutations or deletions of SCF R cause a wide variety of malignancies as well as pigmentation disorders and sterility (5, 6). Mature human SCF R consists of a 499 amino acid (aa) extracellular domain (ECD) with five tandem immunoglobulin-like domains, a 21 aa transmembrane segment, and a 431 aa cytoplasmic domain with the split tyrosine kinase domain (7). Within the ECD, human SCF R shares 73% and 76% aa sequence identity with mouse and rat SCF R, respectively. Alternative splicing of human SCF R generates a potentially secreted isoform that corresponds to the first four Ig-like domains. SCF is expressed as transmembrane and soluble noncovalent homodimers (8). One SCF dimer binds to two molecules of SCF R, inducing receptor dimerization and activation (8). Transmembrane SCF induces more prolonged signaling through SCF R compared to soluble SCF (9). Rat SCF is active on mouse and human cells, but human SCF is only weakly active on mouse cells (10). A 100 kDa glycosylated ECD fragment of SCF R can be shed into the circulation by TACE/ADAM17, and this fragment inhibits the interaction of SCF with transmembrane SCF R (11, 12). SCF is a primary growth and activation factor for mast cells and eosinophils (13). SCF R expression on mast cells enables them to infiltrate SCF-secreting tumors where they promote tumor growth and induce local immune suppression (14). SCF R is up‑regulated on dendritic cells by Th2- or Th17-biasing stimuli, and it is required for subsequent dendritic cell induction of Th2 and Th17 responses (15). SCF R protects vascular smooth muscle cells from apoptosis and assists in the recovery of cardiac function following myocardial infarction (16, 17).

References
  1. Kimura, Y. et al. (2011) PLoS ONE 6:e26918.
  2. Tallini, Y.N. et al. (2009) Proc. Natl. Acad. Sci. USA 106:1808.
  3. Bashamboo, A. et al. (2006) J. Cell Sci. 119:3039.
  4. Sun, L. et al. (2004) J. Clin. Invest. 113:1364.
  5. Pittoni, P. et al. (2011) Oncogene 30:757.
  6. Sartini, S. et al. (2011) Curr. Med. Chem. 18:2893.
  7. Yarden, Y. et al. (1987) EMBO J. 6:3341.
  8. Lemmon, M.A. et al. (1997) J. Biol. Chem. 272:6311.
  9. Miyazawa, K. et al. (1995) Blood 85:641.
  10. Martin, F.H. et al. (1990) Cell 63:203.
  11. Turner, A.M. et al. (1995) Blood 85:2052.
  12. Cruz, A.C. et al. (2004) J. Biol. Chem. 279:5612.
  13. Jamur, M.C. and C. Oliver (2011) Front. Biosci. (School Ed.) 3:1390.
  14. Huang, B. et al. (2008) Blood 112:1269.
  15. Krishnamoorthy, N. et al. (2008) Nat. Med. 14:565.
  16. Wang, C.-H. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:540.
  17. Kanellakis, P. et al. (2006) Cardiovasc. Res. 70:117.
Long Name
Stem Cell Factor Receptor
Entrez Gene IDs
3815 (Human); 16590 (Mouse); 64030 (Rat)
Alternate Names
CD117 antigen; CD117; ckit; c-kit; EC 2.7.10; EC 2.7.10.1; KIT; PBT; piebald trait; Proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; SCF R; SCFR; SCFRmast/stem cell growth factor receptor; soluble KIT variant 1; Tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

Citations for Recombinant Human CD117/c-kit Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Engineering Stem Cell Factor Ligands with Different c-Kit Agonistic Potencies
    Authors: T Tilayov, T Hingaly, Y Greenshpan, S Cohen, B Akabayov, R Gazit, N Papo
    Molecules, 2020-10-21;25(20):.
    Species: Yeast
    Sample Types: Whole Cells
    Applications: Surface Plasmon Resonance
  2. Granulocyte colony-stimulating factor induces the release in the bone marrow of proteases that cleave c-KIT receptor (CD117) from the surface of hematopoietic progenitor cells.
    Authors: Levesque JP, Hendy J, Winkler IG, Takamatsu Y, Simmons PJ
    Exp. Hematol., 2003-02-01;31(2):109-17.
    Applications: Enzyme Assay Substrate

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