Recombinant Human TGF-beta 3 Protein, Animal-Free

R&D Systems | Catalog # BT-TGFB3-AFL

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Key Product Details

Source

E. coli

Accession Number

P10600.1

Applications

Bioactivity
View all TGF-beta 3 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human TGF-beta 3 protein
Ala301-Ser412
Produced using non-animal reagents in an animal-free laboratory.

Purity

>97%, by SDS-PAGE with quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala301

Predicted Molecular Mass

13 kDa

SDS-PAGE

10 kDa, under reducing conditions.

Activity

Measured by its ability to inhibit the IL-4-dependent proliferation of HT‑2 mouse T cells. Tsang, M. et al. (1995) Cytokine 7:389.
The ED50 for this effect is 7.50-75.0 pg/mL.
The specific activity of Recombinant Human TGF-beta 3 is >1.9 x 107 IU/mg, which is calibrated against the Human TGF-beta 3 WHO International Standard (NIBSC code: 09/234).

Scientific Data Images for Recombinant Human TGF-beta 3 Protein, Animal-Free

Equivalent Bioactivity of GMP,Animal-Free, and RUO gradesof Recombinant Human TGF-beta 3.

Equivalent bioactivity of GMP (BT-TGFB3-GMP), Animal-Free (Catalog # BT-TGFB3-AFL) and RUO (BT-TGFB3) grades of Recombinant Human TGF-beta 3 as measured by its ability to inhibit the IL-4-dependent proliferation ofHT‑2 mouse T cells (orange, green, red, respectively).

Animal-Free™ Recombinant Human TGF‑beta 3 Protein Bioactivity.

Animal-Free™ Recombinant Human TGF-beta 3 (Catalog # BT-TGFB3-AFL) inhibits recombinant mouse IL-4 induced proliferation in the HT-2 mouse T cell line. The ED50 for this effect is 7.50-75.0 pg/mL.

Animal-Free™ Recombinant Human TGF‑beta 3 Protein SDS-PAGE.

2 μg/lane of Animal-Free™ Recombinant Human TGF‑beta 3 Protein (Catalog # BT-TGFB3-AFL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 10 kDa under reducing conditions.

Formulation, Preparation, and Storage

BT-TGFB3-AFL
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with Trehalose. 
Reconstitution Reconstitute at 500 μg/mL in 4 mM HCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TGF-beta 3

TGF­ beta 3 (transforming growth factor-beta 3) is a member of a TGF-beta superfamily subgroup that is defined by their structural and functional similarities (1-5). TGF-beta 3 and its closely related proteins, TGF-beta 1 and ­-beta 2, act as cellular switches to regulate immune function, cell proliferation, and epithelial­-mesenchymal transition (4, 6, 7). The non-redundant biological effects of TGF-beta 3 include involvement in palatogenesis, chondrogenesis, and pulmonary development (1, 2, 7-9). Human TGF-­ beta 3 cDNA encodes a 412 amino acid (aa) precursor that contains a 20 aa signal peptide and a 392 aa proprotein. The proprotein is processed by a furin­like convertase to generate a 220 aa latency­associated peptide (LAP) and a 112 aa mature TGF­-beta 3 (10, 11). Mature human TGF­-beta 3 shows 100%, 99%, and 98% aa identity with mouse/dog/horse, rat, and pig TGF-­ beta 3, respectively. TGF-beta 3 is secreted as a complex with LAP. This latent form of TGF-beta 3 becomes active upon cleavage by plasmin, matrix metalloproteases, thrombospondin-1, and a subset of integrins (12). TGF-beta 3 binds with high affinity to TGF-beta RII, a type II serine/threonine kinase receptor. This receptor then phosphorylates and activates type I serine/threonine kinase receptors, TGF-­ beta RI or ALK-­1, to modulate transcription through Smad phosphorylation (13-15). The divergent biological effects exerted by individual TGF-beta isoforms is dependent upon the recruitment of co-receptors (TGF-­ beta RIII and endoglin) and the subsequent initiation of Smad-­dependent or -independent signaling pathways (14, 16, 17).

References

  1. Barrio, M.C. et al. (2014) Cells Tissues Organs. [Epub ahead of print; PMID 24861080].
  2. Doetschman, T. et al. (2012) Genesis 50:59.
  3. Mittl, P.R. et al. (1996) Protein Sci. 5:1261.
  4. Sporn, M.B. (2006) Cytokine Growth Factor Rev. 17:3.
  5. Wahl, S.M. et al. (2006) Immunol. Rev. 213:213.
  6. Chang, H. et al. (2002) Endocr. Rev. 23:787.
  7. Dunker, N. and K. Krieglstein (2000) Eur. J. Biochem. 267:6982.
  8. Jin, J.Z. et al. (2014) Dev. Dyn. [Epub ahead of print; PMID 25104574].
  9. Tang, Q.O. et al. (2009) Expert Opin. Biol Ther. 9:689.
  10. Derynck, R. et al. (1988) EMBO J. 7:3737.
  11. Miyazono, K. et al. (1988) J. Biol. Chem. 263:6407.
  12. Oklu, R. and R. Hesketh (2000) Biochem. J. 352 Pt 3:601.
  13. Cui, X.M. and C.F. Shuler (2000) Int. J. Dev. Biol. 44:397.
  14. de Caestecker, M. (2004) Cytokine Growth Factor Rev. 15:1.
  15. Nakajima, A. et al. (2007) Dev. Dyn. 236:791.
  16. Iwata, J. et al. (2012) J. Clin. Invest. 122:873.
  17. Gatza, C.E. et al. (2010) Cell. Signal. 22:1163.

Long Name

Transforming Growth Factor beta 3

Alternate Names

ARVD1, LDS5, RNHF, TGFB3, TGFbeta 3

Entrez Gene IDs

7043 (Human); 21809 (Mouse); 25717 (Rat)

Gene Symbol

TGFB3

UniProt

P10600.1

Additional TGF-beta 3 Products

Product Documents for Recombinant Human TGF-beta 3 Protein, Animal-Free

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Manufacturing Specifications

Animal-Free Manufacturing Conditions
Our dedicated controlled-access animal-free laboratories ensure that at no point in production are the products exposed to potential contamination by animal components or byproducts. Every stage of manufacturing is conducted in compliance with R&D Systems' stringent Standard Operating Procedures (SOPs). Production and purification procedures use equipment and media that are confirmed animal-free.  

 Production

  • All molecular biology procedures use animal-free media and dedicated labware.
  • Dedicated fermentors are utilized in committed animal-free areas.

Purification

  • Protein purification columns are animal-free.
  • Bulk proteins are filtered using animal-free filters.
  • Purified proteins are stored in animal-free containers in a dedicated cold storage room.

    Quality Assurance

    • Low Endotoxin Level.
    • No impairment of biological activity.
    • High quality product obtained under stringent conditions.
    • For ex vivo research or bioproduction, additional documentation can be provided.

    Please read our complete Animal-Free Statement

    Product Specific Notices for Recombinant Human TGF-beta 3 Protein, Animal-Free

    For research use or further manufacturing only

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