R&D Systems has produced high quality proteins for the research community for more than 35 years. To support developing research and bioproduction demands, we offer products manufactured in a laboratory exclusively dedicated to the production and purification of recombinant proteins under animal-free conditions. Preclinical animal-free proteins are particularly important for researchers concerned with experimental variables caused by trace animal components or mammalian pathogens. Our products generated under animal-free conditions share the same biological activities as those produced using our standard laboratory techniques. Both the preclinical animal-free proteins and the corresponding GMP proteins are manufactured using the same method and have identical expression systems. This ensures a seamless transition from preclinical development to the clinical stage.
Preclinical Animal-Free and GMP Proteins
Protein (Human, Ecoli) | Catalog # Preclinical Animal-Free | Catalog # GMP Animal-Free | DMF Filed For GMP |
---|---|---|---|
Activin A | AFL338 | ||
Betacellulin | BT-BTC-AFL | BT-BTC-GMP | |
BMP-4 | AFL314E | 314E-GMP | |
CD40 Ligand/TNFSF5 aa 108-261 | AFL617 | ||
EGF | AFL236 | ||
FGF acidic/FGF1 | AFL232 | ||
FGF basic/FGF2/bFGF | AFL3718 | 3718-GMP | |
FGF basic/FGF2/bFGF (146 aa) | AFL233 | ||
FGF-4 | AFL235 | ||
Flt-3 Ligand/FLT3L | AFL308E | 308E-GMP | Yes |
G-CSF | AFL214 | ||
GM-CSF | AFL215 | 215-GMP | |
IFN-gamma | AFL285 | 285-GMP | Yes |
IGF-I/IGF-1 | AFL291 | 291-GMP | |
IL-1 beta/IL-1F2 | AFL201 | 201-GMP | |
IL-2 | BT-002-AFL | BT-002-GMP | |
IL-3 | AFL203 | 203-GMP | |
IL-4 | AFL204 | 204-GMP | Yes |
IL-6 | AFL206 | 206-GMP | |
IL-7 | BT-007-AFL | BT-007-GMP | Yes |
IL-15 | BT-015-AFL | BT-015-GMP | Yes |
IL-21 | AFL8879 | 8879-GMP | Yes |
M-CSF | AFL216 | 216-GMP | |
PDGF-AA | AFL221 | 221-GMP | |
PDGF-BB | AFL220 | 220-GMP | |
SCF/c-kit Ligand | AFL255 | 255B-GMP | Yes |
Sonic Hedgehog/Shh | AFL1845 | 1845-GMP | |
Thrombopoietin | AFL288 (Coming Soon) | 288E-GMP | |
TNF-alpha | AFL210 | 210-GMP |

R&D Systems uses dedicated animal-free fermenters in controlled-access labs. This ensures that the products are never exposed to potential contamination by animal components or byproducts.
Our dedicated controlled-access animal-free laboratories ensure that at no point in production are the products exposed to potential contamination by animal products. Every stage of manufacturing is conducted in compliance with R&D Systems stringent Standard Operating Procedures (SOPs). Production and purification procedures use equipment and media that are confirmed animal-free.
R&D Systems uses dedicated animal-free fermenters in controlled-access labs. This ensures that the products are never exposed to potential contamination by animal products.
Production
- All molecular biology procedures use animal-free media and dedicated labware.
- Dedicated fermentors are utilized in committed animal-free areas.
Purification
- Protein purification columns are animal-free.
- Bulk proteins are filtered using animal-free filters.
- Purified proteins are stored in animal-free containers in a dedicated cold storage room.
Quality Assurance
- Endotoxin Level: < 1.0 EU per 1 µg of product.
- No impairment of biological activity.
- High quality product obtained under stringent conditions.
- For ex vivo research or bioproduction, additional documentation can be provided.
Please read our complete Animal-Free Statement
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Bioactivity of Animal-Free Derived IL-1 beta. T helper mouse D10.G4.1 cell proliferation was studied following treatment with the indicated concentrations of animal-free recombinant mouse IL-1 beta (Catalog # AFL401; red line) or recombinant mouse IL-1 beta produced in a typical animal environment (Catalog # 401-ML; brown line). Cell number was assessed by a fluorometric assay using the redox sensitive dye, Resazurin (Catalog # AR002). This data confirms the high bioactivity of our animal-free product and demonstrates that it is comparable to the same molecule produced under typical conditions. |
Related Links:
- Serum-Free and Animal-Free Cell Culture
- Good Manufacturing Practices (GMP)-grade Proteins
- Cell and Gene Therapy Manufacturing
- GMP Small Molecules
- GMP Antibody Services
- Cloudz™ Cell Activation Kits
- GMP Capabilities at Bio-Techne