R&D Systems has produced high quality proteins for the research community for more than 35 years. To support developing research and bioproduction demands, we offer products manufactured in a laboratory exclusively dedicated to the production and purification of recombinant proteins under animal-free conditions. Preclinical animal-free proteins are particularly important for researchers concerned with experimental variables caused by trace animal components or mammalian pathogens. Our products generated under animal-free conditions share the same biological activities as those produced using our standard laboratory techniques. Both the preclinical animal-free proteins and the corresponding GMP proteins are manufactured using the same method and have identical expression systems. This ensures a seamless transition from preclinical development to the clinical stage.
Preclinical Animal-Free and GMP Proteins
|Protein (Human, Ecoli)||Catalog # Preclinical Animal-Free||Catalog # GMP Animal-Free||DMF Filed For GMP|
|CD40 Ligand/TNFSF5 aa 108-261||AFL617|
|FGF basic/FGF2/bFGF (146 aa)||AFL233|
|IL-15 *||AFL247 (Coming Soon)||247-GMP||Yes|
|Thrombopoietin||AFL288 (Coming Soon)||288E-GMP|
GMP ProDots™ Proteins for Cell Therapy Manufacturing
- Closed-processing cytokine addition
- Quickly welds into standard closed-system tubing
- De-risks manufacturing processes
- Improves efficiency of GMP protein supplementation
Animal-Free Manufacturing Conditions
R&D Systems uses dedicated animal-free fermenters in controlled-access labs. This ensures that the products are never exposed to potential contamination by animal components or byproducts.
Our dedicated controlled-access animal-free laboratories ensure that at no point in production are the products exposed to potential contamination by animal products. Every stage of manufacturing is conducted in compliance with R&D Systems stringent Standard Operating Procedures (SOPs). Production and purification procedures use equipment and media that are confirmed animal-free.
R&D Systems uses dedicated animal-free fermenters in controlled-access labs. This ensures that the products are never exposed to potential contamination by animal products.
- All molecular biology procedures use animal-free media and dedicated labware.
- Dedicated fermentors are utilized in committed animal-free areas.
- Protein purification columns are animal-free.
- Bulk proteins are filtered using animal-free filters.
- Purified proteins are stored in animal-free containers in a dedicated cold storage room.
|Bioactivity of Animal-Free Derived IL-1 beta. T helper mouse D10.G4.1 cell proliferation was studied following treatment with the indicated concentrations of animal-free recombinant mouse IL-1 beta (Catalog # AFL401; red line) or recombinant mouse IL-1 beta produced in a typical animal environment (Catalog # 401-ML; brown line). Cell number was assessed by a fluorometric assay using the redox sensitive dye, Resazurin (Catalog # AR002). This data confirms the high bioactivity of our animal-free product and demonstrates that it is comparable to the same molecule produced under typical conditions.
- Good Manufacturing Practices (GMP)-grade Proteins
- Cell and Gene Therapy Manufacturing
- GMP Small Molecules
- GMP Antibody Services
- Cloudz™ Cell Activation Kits
- GMP Capabilities at Bio-Techne