Recombinant Mouse VEGFR2/Flk-1 His-tag Protein, CF

Name: Recombinant Mouse VEGFR2/Flk-1 His-tag Protein, CF
Brand: R&D Systems
SKU: 10095-KD
Price: 474 USD
Availability: InStock

R&D Systems | Catalog # 10095-KD

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Product Specifications
Scientific Data
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

R&D Systems NS0-derived Recombinant Mouse VEGFR2/Flk-1 His-tag Protein (10095-KD)
Quality control testing to verify active proteins with lot specific assays by in-house scientists
All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

P35918

Structure / Form

Monomer

Applications

Bioactivity

View all VEGFR2/KDR/Flk-1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse VEGFR2/KDR/Flk-1 protein
Ala20-Glu762, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala20

Predicted Molecular Mass

844 kDa

SDS-PAGE

120-132 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse VEGFR2/KDR/Flk-1 His-tag (Catalog # 10095-KD) is immobilized at 0.5 μg/mL, 100 μL/well, the concentration of Recombinant Mouse VEGF 164 (Catalog # 493-MV) that produces 50% of the optimal binding response is 0.015-0.12 μg/mL.

Scientific Data Images for Recombinant Mouse VEGFR2/Flk-1 His-tag Protein, CF

Recombinant Mouse VEGFR2/Flk-1 His-tag Protein Binding Activity

When Recombinant Mouse VEGFR2/KDR/Flk-1 (Catalog # 10095-KD) is immobilized at 0.5 μg/mL, 100 μL/well, Recombinant Mouse VEGF 164 (Catalog # 493-MV) binds with an ED₅₀ of 0.015-0.12 μg/mL.

Recombinant Mouse VEGFR2/Flk-1 His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Mouse VEGFR2/KDR/Flk‑1 His-tag (Catalog # 10095-KD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 120-132 kDa.

Formulation, Preparation, and Storage

10095-KD

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: VEGFR2/KDR/Flk-1

VEGFR2 (KDR/Flk-1), VEGFR1 (Flt-1) and VEGFR3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and a tyrosine kinase domains in their intracellular regions. The expression of VEGFR1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis. Mature mouse VEGFR2 is composed of a 743aa residue extracellular domain, a 21 aa residue transmembrane domain and a 584 aa residue cytoplasmic domain. The mature mouse VEGFR2 shares 80% and 93% sequence identity to human and rat VEGFR2. In contrast to VEGFR1 which binds both PlGF and VEGF with high affinity, VEGFR2 binds VEGF but not PlGF with high affinity. The recombinant soluble VEGFR2 binds VEGF with high affinity and is a potent VEGF antagonist (1, 2).