SARS-CoV-2 NSP7 Antibody (1045511)

R&D Systems | Catalog # MAB12505

R&D Systems

Key Product Details

Species Reactivity

SARS-CoV-2

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 1045511
View all NSP7 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived SARS-CoV-2 NSP7 protein
Ser1-Gln83
Accession # YP_009725303.1

Specificity

Detects SARS-CoV-2 NSP7 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for SARS-CoV-2 NSP7 Antibody (1045511)

Detection of SARS-CoV-2 Nsp7 by Western Blot.

Western blot shows recombinant SARS-CoV-2 Nsp7 protein. PVDF membrane was probed with 2 µg/mL of Mouse Anti-SARS-CoV-2 NSP7 Monoclonal Antibody (Catalog # MAB12505) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Nsp7 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Applications for SARS-CoV-2 NSP7 Antibody (1045511)

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: Recombinant SARS-CoV-2 Nsp7 protein

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NSP7

Non-structural protein 7 (NSP7) is one of several functional proteins released by ORF1a-encoded protease cleavage of the pp1a and pp1ab replicase polyproteins expressed from the coronavirus (CoV) genome (1). The NSPs are involved in the replication and transcription of the viral RNA and not incorporated within the virion particles. Coronaviruses include various highly pathogenic strains such as SARS-CoV, MERS-CoV and SARS-CoV-2 that have had significant impact on humans in addition to strains that have negatively impacted livestock. NSP7 is a small 83 amino acid protein that is highly conserved across coronaviruses (1). The NSP7 monomers are composed of a central core N-terminal tri-helical bundle with an additional short C-terminal helix (2). The monomeric units associate to form a large hexadecameric structure with NSP8 (2) where layers of NSP7 fill the spaces in between NSP8 units. The supercomplexes are stacked to form a channel with electrostatic properties that could allow RNA to pass through the channel to facilitate efficient replication and transcription. The NSP7/NSP8 supercomplex was thereby proposed to function as a primase for the viral RNA-dependent RNA polymerase (RdRp), NSP12 (3). In SARS-CoV-2, the RdRp has been shown to have little activity without NSP8/7 as cofactors (4) making NSP7/NSP8 critical for viral polymerase activity. NSP7 has also been shown to interact with several other viral NSP proteins, including NSP5, NSP9, and NSP13 (5) as well as multiple host cell proteins involved in membrane trafficking, signaling, and electron transport including potential drug targets such as COMT and PTGES (6). 

References

  1. Snijder, E.J. et al. (2016) Adv. Virus Res. 96:59.
  2. Zhai, Y. et al. (2005) Nat. Struct. Mol. Bio. 12:980.
  3. Subissi, L. et al. (2014) Antiviral Res. 101:122.
  4. Yin, W. et al. (2020) Science 368:1499.
  5. von Brunn, A. et al. (2007) PLoS One 2:e459.
  6. Gordon, D.E. et al. (2020) Nature 583:459.

Long Name

Non-Structural Protein 7

UniProt

YP_009725303.1

Additional NSP7 Products

Product Documents for SARS-CoV-2 NSP7 Antibody (1045511)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for SARS-CoV-2 NSP7 Antibody (1045511)

For research use only

Related Research Areas

Infectious Diseases

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