SETDB2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-20323

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SETDB2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human SETDB2. The exact sequence is proprietary.

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

82 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SETDB2 Antibody - BSA Free

Western Blot: SETDB2 Antibody [NBP2-20323]

Western Blot: SETDB2 Antibody [NBP2-20323]

Western Blot: SETDB2 Antibody [NBP2-20323] - Sample (30 ug of whole cell lysate) A: SK-N-SH 7. 5% SDS PAGE gel, diluted at 1:1000.
SETDB2 Antibody - BSA Free

Immunoprecipitation: SETDB2 Antibody [NBP2-20323] -

SETDB2 antibody immunoprecipitates SETDB2 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of SETDB2 protein by 2 ug of SETDB2 antibody (NBP2-20323) 7.5% SDS-PAGE The immunoprecipitated SETDB2 protein was detected by SETDB2 antibody (NBP2-20323) diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.
SETDB2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: SETDB2 Antibody [NBP2-20323] -

SETDB2 antibody [N1N2], N-term detects SETDB2 protein at nucleus by immunofluorescent analysis.
Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SETDB2 protein stained by SETDB2 antibody [N1N2], N-term (NBP2-20323) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.

Applications for SETDB2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SETDB2

Proteins that contain a SET domain, such as SETDB2, modulate gene expression epigenetically through histone H3 (see MIM 601128) methylation. SETDB2 is likely a histone H3 methyltransferase, as it contains both the active site and flanking cysteine residues required for catalytic activity (Zhang et al., 2003 [PubMed 12754510]).[supplied by OMIM]

Alternate Names

C13orf4, chromosome 13 open reading frame 4, chronic lymphocytic leukemia deletion region 8, Chronic lymphocytic leukemia deletion region gene 8 protein, CLLD8DKFZp761J1217, DKFZp586I0123, EC 2.1.1.43, histone-lysine N-methyltransferase SETDB2, KMT1FCLLL8, Lysine N-methyltransferase 1F, SET domain bifurcated 2, SET domain, bifurcated 2

Gene Symbol

SETDB2

Additional SETDB2 Products

Product Documents for SETDB2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for SETDB2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Customer Reviews for SETDB2 Antibody - BSA Free

There are currently no reviews for this product. Be the first to review SETDB2 Antibody - BSA Free and earn rewards!

Have you used SETDB2 Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies