Simple Plex Human Flt-3 Ligand/FLT3L Assay Cartridge
R&D Systems | Catalog # SPCKB-PS-009903
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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human Flt-3 Ligand/FLT3L Assay Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Species
Specificity
Cross-reactivity
Interference
精度
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Flt-3 Ligand/FLT3L assay.
Inter-Assay Precision (Precision between assays) Replicates of each Flt-3 Ligand/FLT3L control were tested in multiple assays performed by at least three technicians using two lots of reagents for human Flt-3 Ligand/FLT3L.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 44 | 42 |
| Mean (pg/mL) | 22.9 | 1163 | 22.3 | 1069 |
| Standard Deviation | 0.6 | 60.6 | 2.16 | 95.1 |
| CV% | 2.7 | 5.2 | 9.7 | 8.9 |
Recovery for Simple Plex Human Flt-3 Ligand/FLT3L Assay Cartridge
Recovery for the human Flt-3 Ligand/FLT3L assays was evaluated at three different spiked concentrations of Flt-3 Ligand/FLT3L protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 111 | 110-112 |
Linearity
Samples containing and/or spiked with high concentrations of human Flt-3 Ligand/FLT3L were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Human Flt-3 Ligand/FLT3L Assay Cartridge
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human Flt-3 Ligand/FLT3L Assay. Assay range is 0.16-3868 pg/mL.
Preparation and Storage
Shipping
Stability & Storage
Background: Flt-3 Ligand/FLT3L
Flt-3 Ligand, also known as FLT3L, is a cytokine that promotes the differentiation or expansion of multiple hematopoietic cell lineages including monocytes, immature dendritic cells, and early B cell lineage cells. It synergizes with other cytokines to promote the myeloid differentiation and mobilization of hematopoietic stem cells, to induce NK cell development, and to induce terminal B cell maturation. Flt-3 Ligand/FLT3L is expressed as a dimer by T cells and bone marrow and thymic fibroblasts. Both transmembrane and soluble forms of Flt-3 Ligand/FLT3L signal through the tyrosine kinase receptor Flt-3/Flk-2.
Long Name
Alternate Names
Gene Symbol
Additional Flt-3 Ligand/FLT3L Products
Product Documents for Simple Plex Human Flt-3 Ligand/FLT3L Assay Cartridge
Certificate of Analysis
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Product Specific Notices for Simple Plex Human Flt-3 Ligand/FLT3L Assay Cartridge
For research use only
Related Research Areas
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