SIPA1 Antibody

Novus Biologicals | Catalog # NB100-1390

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all SIPA1 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-ASKQLGSPTADLA corresponding to C-Terminus according to NP_694985.28, NP_006738.3.

Localization

Perinuclear

Specificity

Both variants represent identical protein (NP_694985.28 and NP_006738.3).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for SIPA1 Antibody

Western Blot: SIPA1 Antibody [NB100-1390]

Western Blot: SIPA1 Antibody [NB100-1390]

Western Blot: SIPA1 Antibody [NB100-1390] - Analysis of SIPA1 in Human EBV-immortilized lymphoblastoid lysate (RIPA buffer, 35ug total protein per lane) at 1ug/ml. Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Applications for SIPA1 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 150 kDa band observed in human lysates of EBV-immortilized lymphoblastoid is reported in scientific literature (PMID: 9346962). An additional band of 55-60 kDa band was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when this product is used for other assays than WB.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SIPA1

The product of this gene is a mitogen induced GTPase activating protein (GAP). It exhibits a specific GAP activity for Ras-related regulatory proteins Rap1 and Rap2, but not for Ran or other small GTPases. This protein may also hamper mitogen-induced cell cycle progression when abnormally or prematurely expressed. It is localized to the perinuclear region. Two alternatively spliced variants encoding the same isoform have been characterized to date. [provided by RefSeq]

Alternate Names

GTPase-activating protein Spa-1, MGC102688, MGC17037, p130 SPA-1, signal-induced proliferation-associated 1, signal-induced proliferation-associated protein 1, Sipa-1, SPA1signal-induced proliferation-associated gene 1

Entrez Gene IDs

6494 (Human)

Gene Symbol

SIPA1

UniProt

NP_006738 (Human)

Additional SIPA1 Products

Product Documents for SIPA1 Antibody

Certificate of Analysis

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Product Specific Notices for SIPA1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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