![Western Blot: SKAR Antibody [NBP1-92392]](https://resources.rndsystems.com/images/products/SKAR-Antibody-Western-Blot-NBP1-92392-img0008.jpg "Western Blot: SKAR Antibody [NBP1-92392]")
Loading...
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Loading...
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: SLDELIRKRGAAAKGRLNARPGVGGVRSRVGIQQGLLSQSTRTATFQQRFDARQKIGLSDARLKLGVKDAREKLLQKDARFRIKGKVQDAR
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SKAR Antibody - BSA Free
Western Blot: SKAR Antibody [NBP1-92392]
Western Blot: SKAR Antibody [NBP1-92392] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).![Immunocytochemistry/ Immunofluorescence: SKAR Antibody [NBP1-92392]](https://resources.rndsystems.com/images/products/SKAR-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-92392-img0009.jpg "Immunocytochemistry/ Immunofluorescence: SKAR Antibody [NBP1-92392]")
Immunocytochemistry/ Immunofluorescence: SKAR Antibody [NBP1-92392]
Immunocytochemistry/Immunofluorescence: SKAR Antibody [NBP1-92392] - Staining of human cell line A-431 shows localization to nuclear speckles & cytoplasmic bodies. Antibody staining is shown in green.![SKAR Antibody - BSA Free Immunohistochemistry: SKAR Antibody - BSA Free [NBP1-92392]](https://resources.rndsystems.com/images/products/nbp1-92392_rabbit-polyclonal-skar-antibody-295202516211110.jpg "Immunohistochemistry: SKAR Antibody - BSA Free [NBP1-92392]")
Immunohistochemistry: SKAR Antibody - BSA Free [NBP1-92392]
Staining of human fallopian tube shows moderate nuclear positivity in glandular cells.![SKAR Antibody - BSA Free Western Blot: SKAR Antibody - BSA Free [NBP1-92392]](https://resources.rndsystems.com/images/products/nbp1-92392_rabbit-polyclonal-skar-antibody-29520251633124.jpg "Western Blot: SKAR Antibody - BSA Free [NBP1-92392]")
Western Blot: SKAR Antibody - BSA Free [NBP1-92392]
Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
![SKAR Antibody - BSA Free Immunohistochemistry: SKAR Antibody - BSA Free [NBP1-92392]](https://resources.rndsystems.com/images/products/nbp1-92392_rabbit-polyclonal-skar-antibody-29520251616223.jpg "Immunohistochemistry: SKAR Antibody - BSA Free [NBP1-92392]")
Immunohistochemistry: SKAR Antibody - BSA Free [NBP1-92392]
Staining of human skin shows moderate nuclear positivity in squamous epithelial cells.![SKAR Antibody - BSA Free Western Blot: SKAR Antibody - BSA Free [NBP1-92392]](https://resources.rndsystems.com/images/products/nbp1-92392_rabbit-polyclonal-skar-antibody-30520251016162.jpg "Western Blot: SKAR Antibody - BSA Free [NBP1-92392]")
Western Blot: SKAR Antibody - BSA Free [NBP1-92392]
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251MG sp
![SKAR Antibody - BSA Free Immunohistochemistry: SKAR Antibody - BSA Free [NBP1-92392]](https://resources.rndsystems.com/images/products/nbp1-92392_rabbit-polyclonal-skar-antibody-295202516453417.jpg "Immunohistochemistry: SKAR Antibody - BSA Free [NBP1-92392]")
Immunohistochemistry: SKAR Antibody - BSA Free [NBP1-92392]
Staining of human rectum shows moderate nuclear-cytoplasmic positivity in glandular cells.![SKAR Antibody - BSA Free Immunohistochemistry: SKAR Antibody - BSA Free [NBP1-92392]](https://resources.rndsystems.com/images/products/nbp1-92392_rabbit-polyclonal-skar-antibody-305202575349.jpg "Immunohistochemistry: SKAR Antibody - BSA Free [NBP1-92392]")
Immunohistochemistry: SKAR Antibody - BSA Free [NBP1-92392]
Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.Applications for SKAR Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SKAR
Alternate Names
KIAA1649polymerase delta interacting protein 46, p46, PDIP46S6K1 Aly/REF-like target, polymerase (DNA-directed), delta interacting protein 3, RNA-binding protein P46, SKARpolymerase delta-interacting protein 3,46 kDa DNA polymerase delta interaction protein
Gene Symbol
POLDIP3
Additional SKAR Products
Product Documents for SKAR Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for SKAR Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Customer Reviews for SKAR Antibody - BSA Free
There are currently no reviews for this product. Be the first to review SKAR Antibody - BSA Free and earn rewards!
Have you used SKAR Antibody - BSA Free?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...