SMN Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-13414

Novus Biologicals
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Key Product Details

Species Reactivity

Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of mouse SMN. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for SMN Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: SMN Antibody [NBP3-13414]

Immunocytochemistry/ Immunofluorescence: SMN Antibody [NBP3-13414]

Immunocytochemistry/Immunofluorescence: SMN Antibody [NBP3-13414] - SMN antibody detects SMN protein at cytoplasm by immunofluorescent analysis. Sample: Neuro2A cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SMN stained by SMN antibody (NBP3-13414) diluted at 1:500.
SMN Antibody

Western Blot: SMN Antibody [NBP3-13414] -

Western Blot: SMN Antibody [NBP3-13414] - Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with SMN (NBP3-13414) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
SMN Antibody

Western Blot: SMN Antibody [NBP3-13414] -

Western Blot: SMN Antibody [NBP3-13414] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with SMN antibody (NBP3-13414) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for SMN Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SMN

SMN (Gemin 1) is the central component of a large oligomeric complex (SMN complex), including Gemins 2-7, that is necessary and sufficient for the in vivo assembly of Sm proteins onto the small nuclear (sn)RNAs that mediate pre-mRNA splicing. The SMN complex is found in the cytoplasm and nucleus. Within the nucleus, this protein localizes to subnuclear bodies called gems which are found near coiled bodies containing high concentrations of small ribonucleoproteins (snRNPs). Spinal muscular atrophy, a motor neuron disorder, results from reduced levels or a mutation in the Gemin 1 protein.

Alternate Names

Gemin-1, Kugelberg-Welander disease), SMNC, survival motor neuron protein, survival of motor neuron 1, telomeric

Gene Symbol

SMN1

Additional SMN Products

Product Documents for SMN Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for SMN Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for SMN Antibody - BSA Free

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  • Q: Could you recommond the Human specific SMN antibody? It will only recognize human SMN, without cross reaction with mouse smn.

    A: We do not have an SMN antibody that has been validated NOT to work in mouse samples. When an antibody is found to not react with a certain species, we mark the data sheet with a (-) next to the species' name. Unfortunately, the SMN antibodies that do not list mouse have not been tested in mouse, and may react.

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