SOX4 Antibody (4A1E8)

Novus Biologicals | Catalog # NBP3-33358

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 4A1E8
View all SOX4 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 350-450 of human SOX4 (NP_003098.1).

Sequence:
AAGRSPADHRGYASLRAASPAPSSAPSHASSSASSHSSSSSSSGSSSSDDEFEDDLLDLNPSSNFESMSLGSFSSSSALDRDLDFNFEPGSGSHFEFPDYC

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SOX4 Antibody (4A1E8)

SOX4 Antibody (4A1E8)

Western Blot: SOX4 Antibody (4A1E8) [NBP3-33358] -

Western Blot: SOX4 Antibody (4A1E8) [NBP3-33358] - Western blot analysis of various lysates, using SOX4 Rabbit mAb at 1:20000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
SOX4 Antibody (4A1E8)

Western Blot: SOX4 Antibody (4A1E8) [NBP3-33358] -

Western Blot: SOX4 Antibody (4A1E8) [NBP3-33358] - Western blot analysis of lysates from Rat lung, using SOX4 Rabbit mAb at 1:20000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
SOX4 Antibody (4A1E8)

Western Blot: SOX4 Antibody (4A1E8) [NBP3-33358] -

Western Blot: SOX4 Antibody (4A1E8) [NBP3-33358] - Western blot analysis of lysates from wild type (WT) and SOX4 knockdown (KD) 293T cells using SOX4 Rabbit mAb at 1:5000 dilution incubated overnight at 4C.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

Applications for SOX4 Antibody (4A1E8)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 ug/mL

Western Blot

1:2000 - 1:20000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SOX4

SOX4 encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins, such as syndecan binding protein (syntenin). The protein may function in the apoptosis pathway leading to cell death as well as to tumorigenesis and may mediate downstream effects of parathyroid hormone (PTH) and PTH-related protein (PTHrP) in bone development. The solution structure has been resolved for the HMG-box of a similar mouse protein.

Alternate Names

ecotropic viral integration site 16, EVI16, SRY (sex determining region Y)-box 4, SRY-related HMG-box gene 4, transcription factor SOX-4

Gene Symbol

SOX4

Additional SOX4 Products

Product Documents for SOX4 Antibody (4A1E8)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for SOX4 Antibody (4A1E8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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