SPANXB1 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # H00728695-B01P

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Mouse IgG

Format

Azide and BSA Free
View all SPANXB1 Primary Antibodies »

Product Specifications

Immunogen

SPANXB1 (NP_115850.1, 1 a.a. - 103 a.a.) full-length human protein. MGQQSSVRRLKRSVPCESNEANEANEANKTMPETPTGDSDPQPAPKKMKTSESSTILVVRYRRNVKRTSPEELVNDHARENRINPDQMEEEEFIEITTERPKK

Specificity

Reacts with SPANX family, member B1.

Clonality

Polyclonal

Host

Mouse

Isotype

IgG

Scientific Data Images for SPANXB1 Antibody - Azide and BSA Free

Western Blot: SPANXB1 Antibody [H00728695-B01P]

Western Blot: SPANXB1 Antibody [H00728695-B01P]

Western Blot: SPANXB1 Antibody [H00728695-B01P] - Analysis of SPANXB1 expression in transfected 293T cell line by SPANXB1 polyclonal antibody. Lane 1: SPANXB1 transfected lysate(11.33 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: SPANXB1 Antibody [H00728695-B01P]

Immunocytochemistry/ Immunofluorescence: SPANXB1 Antibody [H00728695-B01P]

Immunocytochemistry/Immunofluorescence: SPANXB1 Antibody [H00728695-B01P] - Analysis of purified antibody to SPANXB1 on Hs 181.Tes cell. (antibody concentration 10 ug/ml)

Applications for SPANXB1 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Western Blot

1:500
Application Notes
This antibody is reactive against transfected lysate in western blot, immunofluorescence, and as a detection antibody in ELISA.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SPANXB1

Temporally regulated transcription and translation of several testis-specific genes is required to initiate the series of molecular and morphological changes in the male germ cell lineage necessary for the formation of mature spermatozoa. This gene is a member of the SPANX family of cancer/testis-associated genes, which are located in a cluster on chromosome X. The SPANX genes encode differentially expressed testis-specific proteins that localize to various subcellular compartments. This particular gene maps to chromosome X in a head-to-tail orientation with SPANX family member B2, which appears to be a duplication of the B1 locus. The SPANXB genes are unique members of this gene family, since they contain an additional 18 nt in their coding region compared to the majority of family members. Although the protein encoded by this gene contains consensus nuclear localization signals, the major site for subcellular localization of expressed protein is in the cytoplasmic droplets of ejaculated spermatozoa. This protein provides a biochemical marker for studying the unique structures in spermatazoa, while attempting to further define its role in spermatogenesis. [provided by RefSeq]

Alternate Names

B1, C, Cancer/testis antigen 11.2, cancer/testis antigen family 11, member 2, CT11.1, CT11.2, CT11.2B1, CT11.3, CT11.4, CTp11, dJ171K16.1, NAP-X, Nuclear-associated protein SPAN-Xb, Nuclear-associated protein SPAN-Xf, SPANX, SPANX family member B/F, SPANX family, member B1, SPANXA, SPAN-Xa, SPANX-A, SPANXB, SPAN-Xb, SPANX-B, SPANXB2, SPANX-C, SPANX-D, SPANXE SPANX family, member E, SPAN-Xf, SPANX-F, SPANXF1, sperm protein associated with the nucleus on the X chromosome B/F, sperm protein associated with the nucleus, X chromosome, family member B1

Entrez Gene IDs

728695 (Human)

Gene Symbol

SPANXB1

UniProt

NP_115850.1 (Human)

Additional SPANXB1 Products

Product Documents for SPANXB1 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for SPANXB1 Antibody - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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