STAT5A [Methyl Arg31] Antibody - BSA Free

Name: STAT5A [Methyl Arg31] Antibody - BSA Free
Brand: Novus Biologicals
SKU: NBP2-44184
Price: 619 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-44184

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

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Product Specifications

Immunogen

STAT5A [Methyl Arg31] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic monomethylated peptide surrounding Arginine 31 of human STAT. (Uniprot: P42229)

Reactivity Notes

A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and rat. Cross-reactivity with STAT5 from other sources has not been determined.

Modification

Methyl Arg31

Localization

Cytoplasm, Nucleus (upon phosphorylation at Tyr694)

Specificity

This antibody is specific for STAT5 R31/Me1 and does not reacts with other STATs surrounding aa31.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for STAT5A [Methyl Arg31] Antibody - BSA Free

Western Blot: STAT5A [Methyl Arg31] Antibody [NBP2-44184]

Western Blot: STAT5A [Methyl Arg31] Antibody [NBP2-44184] - Antigen: non-modified, monomethylated and asymmetric dimethylated forms of the immunizing peptide. Load: 100, 10, or 1 picomolar as indicated.

STAT5A [Methyl Arg31] Antibody

Dot blot of Rabbit Anti-STAT5 R31-Me1 antibody. Antigen: non-modified, monomethylated and asymmetric dimethylated forms of the immunizing peptide. Load: 100, 10, or 1 picomolar as indicated. Primary antibody: STAT5 R31-Me1 antibody at 1:1000 for 90 min at RT. (Date: 10/22/14; Exposure time: 12.5 seconds)

Dot Blot: STAT5A [Methyl Arg31] Antibody - BSA Free [NBP2-44184] -

Dot blot of STAT5A [Methyl Arg31] Antibody - BSA Free. Antigen: non-modified, monomethylated and asymmetric dimethylated forms of the immunizing peptide. Load: 100, 10, or 1 picomolar as indicated. Primary antibody: STAT5A [Methyl Arg31] Antibody - BSA Free at 1:1000 for 90 min at RT. (Date: 10/22/14; Exposure time: 12.5 seconds)

Applications for STAT5A [Methyl Arg31] Antibody - BSA Free

Application

Recommended Usage

ELISA

1:5000

Western Blot

1:500

Application Notes

This product has been tested by Dot Blot and is useful for Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~90.6 kDa corresponding to STAT5 protein by Western Blotting in the appropriate cell lysate or extract.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 30% Glycerol

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: STAT5a

Stat5 is part of a 7 protein family known as signal transducer and activator of transcription (STAT) which contributes to signal transduction by cytokine, hormone and growth factor (1). Signal transducer and activator of transcription 5 (Stat5) functions both in signal transduction and activation of transcription. It has been found that cytoplasmic Stat5 is translocated to the nucleus in response to phosphorylation. Stat5 225; tyrosine phosphorylation is activated predominantly by IL2 but also by IL-3, IL-5, IL-7, IL-9, IL-15, G-CSF andGM-CSF(2). Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity. NCoA-1/SRC-1 acts as a coactivator for both the alpha- and beta-isoforms of Stat5 (3).

Long Name

Signal Transducer and Activator of Transcription 5

Alternate Names

MGF, signal transducer and activator of transcription 5A, STAT5

Gene Symbol

STAT5A

UniProt

P42229 (Human)

Additional STAT5a Products

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Product Specific Notices for STAT5A [Methyl Arg31] Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.