SUG1 Antibody (25D5) - Azide and BSA Free

Novus Biologicals | Catalog # NB100-345

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Knockdown Validated

Cited:

Western Blot, Immunoprecipitation, Chemotaxis

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 25D5

Format

Azide and BSA Free
View all SUG1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encoding full-length SUG1 purified from E. coli.

Localization

Cytoplasmic

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SUG1 Antibody (25D5) - Azide and BSA Free

Western Blot: SUG1 Antibody (25D5) [NB100-345]

Western Blot: SUG1 Antibody (25D5) [NB100-345]

Western Blot: SUG1 Antibody (25D5) [NB100-345] - SUG1 antibody with 30 ug whole cell lysate run on SDS-PAGE. Lane A: 293T; B: A431; C: H1299; D: Hela; E: Hep G2; F: Molt-4; G: Raji 10% SDS PAGE diluted at 1:1000
Knockdown Validated: SUG1 Antibody (25D5) [NB100-345]

Western Blot: SUG1 Antibody (25D5) [NB100-345]

SUG1-Antibody-25D5-Knockdown-Validated-NB100-345-img0003.jpg
SUG1 Antibody (25D5) - Azide and BSA Free

Western Blot: SUG1 Antibody (25D5) [NB100-345] -

Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with SUG1 antibody [25D5] (NB100-345) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Applications for SUG1 Antibody (25D5) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:1000
Application Notes
IP reactivity reported in (PMID: 19660582). SUG1 antibody validated for CHIP from a verified customer review.

Reviewed Applications

Read 2 reviews rated 4 using NB100-345 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS, 20% Glycerol

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SUG1

Human SUG1 (also known as p45, Rpt6 and Cim3) is a component of the 19S regulatory subunit of the 26S proteosome complex. It is one of the AAA ATPas found in the 19S regulatory complex that is thought to participate in the unfolding of ubiquinated proteins in an ATP-dependent manner. It has also been shown to directly interact with the nucleotide excision repair protein XPB.

Alternate Names

MSUG1 protein, p45, p45/SUG26S protease regulatory subunit 8, proteasome (prosome, macropain) 26S subunit, ATPase, 5, proteasome 26S ATPase subunit 5, Proteasome 26S subunit ATPase 5, Proteasome subunit p45, S8, SUG-1, SUG126S proteasome AAA-ATPase subunit RPT6, Tat-binding protein homolog 10, TBP10, thyroid receptor interactor 1, TRIP1Thyroid hormone receptor-interacting protein 1

Entrez Gene IDs

5705 (Human)

Gene Symbol

PSMC5

UniProt

P62195 (Human)

Additional SUG1 Products

Product Documents for SUG1 Antibody (25D5) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for SUG1 Antibody (25D5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SUG1 Antibody (25D5) - Azide and BSA Free

Customer Reviews for SUG1 Antibody (25D5) - Azide and BSA Free (2)

4 out of 5
2 Customer Ratings
5 Stars
50%
4 Stars
0%
3 Stars
50%
2 Stars
0%
1 Stars
0%

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Customer Images

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested:
    Species: Human
    Verified Customer | Posted 05/07/2013
    50ug protein from WCEs from SVG-A cells. Untreated, Scr control and PSMC5 siRNA treated (x3)
    SUG1 Antibody (25D5) - Azide and BSA Free NB100-345
  • Name: Anonymous
    Application: Chromatin Immunoprecipitation
    Sample Tested: Human Whole Cell lysate
    Species: Human
    Verified Customer | Posted 03/02/2010

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