TGF-beta 2 Antibody

Name: TGF-beta 2 Antibody
Brand: R&D Systems
SKU: AF-302-NA
Price: 489 USD
Availability: InStock

R&D Systems | Catalog # AF-302-NA

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Citations (8)

Product Documents

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Multi-Species

Cited:

Human, Mouse, Canine

Applications

Validated:

Western Blot, Neutralization

Cited:

Neutralization

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all TGF-beta 2 Primary Antibodies »

Product Specifications

Immunogen

Porcine platelet-derived TGF-beta 2

Specificity

Detects TGF‑ beta 2 in direct ELISAs and Western blots. In direct ELISAs and Western blots (non-reducing conditions), less than 2% cross‑reactivity with TGF-beta 1, TGF-beta 3, and TGF-beta 5 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for TGF-beta 2 Antibody

TGF‑ beta 2 Inhibition of IL‑4-dependent Cell Proliferation and Neutralization by TGF‑ beta 2/Antibody.

Porcine TGF-beta 2 (Catalog # 102-B2) inhibits Recombinant Mouse IL-4 (Catalog # 404-ML) induced proliferation in the HT-2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse IL-4 (7.5 ng/mL) activity elicited by Porcine TGF-beta 2 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-TGF-beta 2/Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-302-NA). The ND₅₀ is typically 0.0100-0.100 µg/mL.

Human TGF-beta 2 ELISA Standard Curve

Recombinant Human TGF‑ beta 2 (Catalog # 302-B2) was serially diluted and captured by Mouse Anti-TGF‑ beta 2 Monoclonal Antibody (Catalog # MAB612) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-TGF‑ beta 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-302-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for TGF-beta 2 Antibody

Application

Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human TGF-beta 2 (Catalog # 302-B2)

Neutralization

Measured by its ability to neutralize TGF‑ beta 2 inhibition of IL‑4-dependent proliferation in the HT‑2 mouse T cell line. Tsang, M. et al. (1995) Cytokine 7:389. The Neutralization Dose (ND₅₀) is typically 0.0100-0.100 µg/mL in the presence of 1 ng/mL Porcine TGF‑ beta 2 and 7.5 ng/mL Recombinant Mouse IL‑4.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: TGF-beta 2

TGF- beta 2 (transforming growth factor beta 2) is a highly pleiotropic cytokine with important functions during cardiac, lung, craniofacial, limb, eye, ear, and urogenital system development. It forms a latent complex with LAP and one of the LTBP TGF-beta binding proteins. The active protein is released by Plasmin, MMP, Thrombospondin 1, or Integrin action. TGF-beta 2 signals through TGF-beta RII, Betaglycan, and either TGF-beta RI/ALK-5 or ALK-1.