TRF-1 Antibody (4E4) - Azide and BSA Free

Novus Biologicals | Catalog # NBP1-41217

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4E4

Format

Azide and BSA Free
View all TRF-1 Primary Antibodies »

Product Specifications

Immunogen

The exact sequence is proprietary.

Localization

Nuclear

Marker

Telomere Marker

Specificity

This antibody will recognize human TRF1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TRF-1 Antibody (4E4) - Azide and BSA Free

TRF-1 Antibody (4E4) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: TRF-1 Antibody (4E4) [NBP1-41217] -

TRF1 antibody [4E4] detects TRF1 protein at telomere by immunofluorescent analysis.
Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TRF1 protein stained by TRF1 antibody [4E4] (NBP1-41217) diluted at 1:500.
Blue: Hoechst 33342 staining.
TRF-1 Antibody (4E4) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: TRF-1 Antibody (4E4) [NBP1-41217] -

Applications for TRF-1 Antibody (4E4) - Azide and BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

Assay dependent

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunoprecipitation

Assay dependent

Western Blot

Assay dependent

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TRF-1

TRF1 binds the double-stranded telomeric repeat sequence, TTAGGG. TRF1 and TRF2 are key components of vertebrate telomeres and bind to double-stranded telomeric DNA as homodimers. Dimerization involves the TRF homology (TRFH) domain, which also mediates interactions with other telomeric proteins. Despite a modest sequence identity, the 2 TRFH domains have the same entirely alpha-helical architecture, resembling a twisted horseshoe. The dimerization interfaces feature unique interactions that prevent heterodimerization. Mutational analysis of TRF1 corroborated the structural data and underscored the importance of the TRFH domain in dimerization, DNA binding, and telomere localization.

Long Name

Telomeric Repeat Binding Factor

Alternate Names

PIN-2, TERF1, TRF1

Entrez Gene IDs

7013 (Human)

Gene Symbol

TERF1

Additional TRF-1 Products

Product Documents for TRF-1 Antibody (4E4) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for TRF-1 Antibody (4E4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TRF-1 Antibody (4E4) - Azide and BSA Free

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Protocols

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FAQs

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