UAP1 Antibody (5S7M2)

Novus Biologicals | Catalog # NBP3-16671

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5S7M2 expressed in HEK293
View all UAP1 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 400-500 of human UAP1 (Q16222). EDEFSPLKNADSQNGKDNPTTARHALMSLHHCWVLNAGGHFIDENGSRLPAIPRSATNGKSETITADVNHNLKDANDVPIQCEISPLISYAGEGLESYVAD

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for UAP1 Antibody (5S7M2)

Western Blot: UAP1 Antibody (5S7M2) [NBP3-16671]

Western Blot: UAP1 Antibody (5S7M2) [NBP3-16671]

Western Blot: UAP1 Antibody (5S7M2) [NBP3-16671] - Western blot analysis of extracts of various cell lines, using UAP1 Rabbit mAb (NBP3-16671) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunocytochemistry/ Immunofluorescence: UAP1 Antibody (5S7M2) [NBP3-16671]

Immunocytochemistry/ Immunofluorescence: UAP1 Antibody (5S7M2) [NBP3-16671]

Immunocytochemistry/Immunofluorescence: UAP1 Antibody (5S7M2) [NBP3-16671] - Immunofluorescence analysis of U-2 OS cells using UAP1 Rabbit mAb (NBP3-16671) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: UAP1 Antibody (5S7M2) [NBP3-16671]

Immunocytochemistry/ Immunofluorescence: UAP1 Antibody (5S7M2) [NBP3-16671]

Immunocytochemistry/Immunofluorescence: UAP1 Antibody (5S7M2) [NBP3-16671] - Immunofluorescence analysis of C6 cells using UAP1 Rabbit mAb (NBP3-16671) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Applications for UAP1 Antibody (5S7M2)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: UAP1

Converts UDP and GlcNAc-1-P into UDP-GlcNAc, and UDP and GalNAc-1-P into UDP-GalNAc. Isoform AGX1 has 2 to 3 times higher activity towards GalNAc-1-P, while isoform AGX2 has 8 times more activity towards GlcNAc-1-P

Alternate Names

AgX, AGX1, AGX2, Antigen X, EC 2.7.7, EC 2.7.7.23, EC 2.7.7.27, SPAG2, sperm associated antigen 2, Sperm-associated antigen 2, UDP-N-acetylhexosamine pyrophosphorylase, UDP-N-acteylglucosamine pyrophosphorylase 1

Gene Symbol

UAP1

Additional UAP1 Products

Product Documents for UAP1 Antibody (5S7M2)

Certificate of Analysis

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Product Specific Notices for UAP1 Antibody (5S7M2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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