Ubiquitin-activating Enzyme/UBE1 Antibody (05) [CoraFluor™ 1]

Name: Ubiquitin-activating Enzyme/UBE1 Antibody (05) [CoraFluor™ 1]
Brand: Novus Biologicals
SKU: NBP3-05789CL1
Price: 739 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-05789CL1

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunoprecipitation

Label

CoraFluor 1

Antibody Source

Monoclonal Mouse IgG₁ Clone # 05

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Product Specifications

Immunogen

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Ubiquitin-activating Enzyme/UBE1 (Accession#: NP_003325.2; Ser2-Arg1058).

Reactivity Notes

No cross-reactivity in ELISA with:

Insect cell lysate

Specificity

No cross-reactivity in ELISA with: Insect cell lysate

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Description

This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.

For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.

Scientific Data Images for Ubiquitin-activating Enzyme/UBE1 Antibody (05) [CoraFluor™ 1]

Product Feature: CoraFluor Probes for TR-FRET

CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.

Applications for Ubiquitin-activating Enzyme/UBE1 Antibody (05) [CoraFluor™ 1]

Application

Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Recommended applications are based on validated applications from the unconjugated base product NBP3-05789. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: Ubiquitin-activating Enzyme/UBE1

E1 Ubiquitin Activating Enzyme is encoded by this gene catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. Alternatively spliced transcript variants that encode the same protein have been described.

Alternate Names

A1S9T, AMCX1, GXP1, POC20, SMAX2, UBA1, UBE1, Ubiquitinactivating Enzyme

Gene Symbol

UBA1

Additional Ubiquitin-activating Enzyme/UBE1 Products

Product Documents for Ubiquitin-activating Enzyme/UBE1 Antibody (05) [CoraFluor™ 1]

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Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Ubiquitin-activating Enzyme/UBE1 Antibody (05) [CoraFluor™ 1]

Sold for research purposes only under agreement from Massachusetts General Hospital, US Patent No. 12,134,721. A license is required for development of new commercial assay platforms (e.g., microarrays, microfluidics), diagnostic use or other unlicensed uses. Please contact Technical Support for further details: techsupport@bio-techne.com.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

E1 Ubiquitin/Ubl Activating Enzymes

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