V5 Epitope Tag Antibody (1071) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-43626

Novus Biologicals
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Key Product Details

Species Reactivity

Epitope Tag

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1071

Format

Azide and BSA Free
View all V5 Epitope Tag Primary Antibodies »

Product Specifications

Immunogen

The immunogen used to generate this antibody corresponds to V5 Epitope Tag

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for V5 Epitope Tag Antibody (1071) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: V5 Epitope Tag Antibody (1071) [NBP2-43626]

Immunocytochemistry/ Immunofluorescence: V5 Epitope Tag Antibody (1071) [NBP2-43626]

Immunocytochemistry/Immunofluorescence: V5 Epitope Tag Antibody (1071) [NBP2-43626] - V5-tag tagged protein transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: V5 tag stained by V5 tag antibody [1071] diluted at 1:500.Blue: Hoechst 33342 staining.
Immunoprecipitation: V5 Epitope Tag Antibody (1071) [NBP2-43626]

Immunoprecipitation: V5 Epitope Tag Antibody (1071) [NBP2-43626]

Immunoprecipitation: V5 Epitope Tag Antibody (1071) [NBP2-43626] - Analysis of 400 ug cell lysate/extract of V5-fused gene transfected 293T cells A. 30 ug lysate/extract of transfected 293T cell B. Control with 2 ug of preimmune mouse IgG C. Immunoprecipitation of V5-fused protein by 2 ug of V5 tag antibody [1071] 12% SDS-PAGE The immunoprecipitated V5-fused protein was detected by V5 tag antibody diluted at 1:1000. EasyBlot anti-mouse IgG was used as a secondary reagent.
V5 Epitope Tag Antibody (1071) - Azide and BSA Free

Western Blot: V5 Epitope Tag Antibody (1071) [NBP2-43626] -

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with V5 tag antibody [GT1071] (NBP2-43626) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Applications for V5 Epitope Tag Antibody (1071) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:2000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: V5 Epitope Tag

The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).

Alternate Names

GKPIPNPLLGLDST Epitope Tag, Parainfluenza virus 5 V/P, Paramyxovirus SV5

Additional V5 Epitope Tag Products

Product Documents for V5 Epitope Tag Antibody (1071) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for V5 Epitope Tag Antibody (1071) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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