Viral HIV-1 Gag p24 Antibody (1042942)

R&D Systems | Catalog # MAB11189

R&D Systems

Key Product Details

Species Reactivity

Viral

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1042942
View all HIV-1 Gag p24 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant viral HIV-1 Gag p24
Pro149-Gly354
Accession # Q77YG1_9HIV1

Specificity

Detects viral HIV-1 Gag p24 in direct ELISAs

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Viral HIV-1 Gag p24 Antibody (1042942)

Detection of Recombinant HIV-1 Gag p24 HIV‑1 Gag p24 by Western Blot.

Western blot shows lysates of rHIV-1 Gag p24. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Viral HIV‑1 Gag p24 Monoclonal Antibody (Catalog # MAB11189) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for HIV‑1 Gag p24 at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Applications for Viral HIV-1 Gag p24 Antibody (1042942)

Application
Recommended Usage

ELISA

This antibody functions as an ELISA capture antibody when paired with mouse anti-HIV‑1 Gag p24 Monoclonal Antibody (Catalog # MAB11188).This product is intended for assay development on various assay platforms requiring antibody pairs.

Western Blot

1 µg/mL
Sample: rHIV-1 Gag p24

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HIV-1 Gag p24

The gag gene of human immunodeficiency virus 1 (HIV-1) encodes a precursor protein known as Pr55Gag. The viral protease PR cleaves this precursor to generate p17, p24, p7, and p6 proteins which are required for virus particle assembly. p24 is a major viral core structural protein. Its measurement is commonly used as an indicator of HIV-1 infection and viral load.

Long Name

Human Immunodeficiency Virus Type 1 GAG/Group-specific Antigen core protein of 24 kDa

Alternate Names

CA, Capsid Protein, HIV1 Gag p24, Pr55(Gag)

Entrez Gene IDs

155030 (Viral)

Gene Symbol

GAG

UniProt

Q77YG1_9HIV1

Additional HIV-1 Gag p24 Products

Product Documents for Viral HIV-1 Gag p24 Antibody (1042942)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Viral HIV-1 Gag p24 Antibody (1042942)

For research use only

Related Research Areas

Infectious Diseases

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