West Nile Virus Envelope Antibody (GT3029) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-13574
![Western Blot: West Nile Virus Envelope Antibody (GT3029) [NBP3-13574]](https://resources.rndsystems.com/images/products/West-Nile-Virus-Envelope-Antibody-GT3029-Western-Blot-NBP3-13574-img0004.jpg "Western Blot: West Nile Virus Envelope Antibody (GT3029) [NBP3-13574]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
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Key Product Details
Species Reactivity
Virus
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # GT3029
Format
Azide and BSA Free
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of West Nile Virus Envelope protein (West Nile virus (strain NY99-IC)). The exact sequence is proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Centrifuge briefly prior to opening.
Scientific Data Images for West Nile Virus Envelope Antibody (GT3029) - Azide and BSA Free
Western Blot: West Nile Virus Envelope Antibody (GT3029) [NBP3-13574]
Western Blot: West Nile Virus Envelope Antibody (GT3029) [NBP3-13574] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with West Nile Virus Envelope protein antibody [GT3029] (NBP3-13574) diluted at 1:1000.![Immunocytochemistry/ Immunofluorescence: West Nile Virus Envelope Antibody (GT3029) [NBP3-13574]](https://resources.rndsystems.com/images/products/West-Nile-Virus-Envelope-Antibody-GT3029-Immunocytochemistry-Immunofluorescence-NBP3-13574-img0002.jpg "Immunocytochemistry/ Immunofluorescence: West Nile Virus Envelope Antibody (GT3029) [NBP3-13574]")
Immunocytochemistry/ Immunofluorescence: West Nile Virus Envelope Antibody (GT3029) [NBP3-13574]
Immunocytochemistry/Immunofluorescence: West Nile Virus Envelope Antibody (GT3029) [NBP3-13574] - West Nile Virus Protein E protein antibody [GT3029] detects West Nile Virus Protein E protein at cytoplasm by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: West Nile Virus Protein E protein stained by West Nile Virus Protein E protein antibody [GT3029] (NBP3-13574) diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar= 10 um.Applications for West Nile Virus Envelope Antibody (GT3029) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Product Documents for West Nile Virus Envelope Antibody (GT3029) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for West Nile Virus Envelope Antibody (GT3029) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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