Webinar: Utilizing Peptide Mapping for Deeper Characterization of Biotherapeutic Charge Variants

Watch our webinar on demand “Utilizing Peptide Mapping for Deeper Characterization of Biotherapeutic Charge Variants.” 

Discover how imaged capillary isoelectric focusing (icIEF) fractionation combined with mass spectrometry (MS) enables in-depth characterization of charge heterogeneity in monoclonal antibodies (mAbs) and novel biotherapeutics.  

Our first speaker, Julien Bourquin (Merck KGaA), describes how icIEF fractionation combined with peptide mapping (LC-MS) can link individual charge variants to their underlying post-translational modifications at specific sites.  

Dr. Laurence Talbot (Bio-Techne) highlights the latest advancements in icIEF-fractionation. She will give an overview of the different downstream characterization methods for charge variant characterization, including LC-MS (intact mass analysis), SPR and mass photometry and the important insights these methods can provide on charge variants.

The webinar with cover the following topics: 

• A streamlined workflow combining charge variant fractionation with peptide mapping (LC-MS) to determine underlying post-translational modifications at specific sites in mAbs
• A robust, scalable, and MS platform-independent analytical strategy for addressing charge heterogeneity challenges in complex biologics using the MauriceFlex system.
• An improved understanding of critical quality attributes to accelerate development timelines and strengthen regulatory submissions