Milo Single Cell Western Blot System

Milo is a Single-Cell Western platform that’ll let you measure protein expression in thousands of single cells in a single run. You’ll get it all done in 4–6 hours with no overnight transfer step. And you can use off-the-shelf primary Western antibodies too — try that with flow cytometry! Milo measures protein expression heterogeneity and identifies cell subpopulations so you can make measurements you can’t make any other way and uncover novel insights about your samples!

Understand protein expression heterogeneity in complex samples using single-cell western analysis

Milo measures protein expression in ~1,000 single-cells in one 4-hour scWestern experiment so you can quantify heterogeneity of your target at the single-cell level to determine expression patterns. Using antibodies that work for traditional western blot, Milo provides a platform to analyze single-cell proteomics to determine the percentage of cells in your sample that are target positive.

Validate single-cell RNA-seq Data

Use conventional western blot antibodies to validate your single-cell RNA-seq data with single-cell protein data using our scWestern instrument Milo. Plus, scWest chips can be archived for up to 9 months after you run them so you have plenty of time to get your RNA sequencing results back before you have to probe for your targets of interest for single-cell proteomics data. 

Simplify phospho-flow signaling studies 

Eliminate the fixation and permeabilization steps of flow cytometry! Milo chemically lyses the cells captured on the scWest chip before analysis to gain access to intracellular and intranuclear compartments more easily than with flow cytometry. By lysing the cells, Milo can detect challenging proteins like transcription factors and even methylated histones!

You can also use Milo's sizing step to separate out non-specific binding, which is more common with phospho-specific antibodies and cannot be resolved with flow cytometry methods.

Detect proteins that lack good flow cytometry antibodies

Milo is an open, automated single-cell western platform where you can use the large commercial catalog of western blot validated antibodies, which is 10-100x larger than the flow cytometry antibody catalog. Don't get stuck without a flow antibody against your target of interest again. Use Milo's Single-Cell Western technology to perform single-cell protein analysis.

Measure protein isoform heterogeneity

Milo's molecular weight sizing step can resolve protein isoforms that differ in molecular weight, allowing you to measure how many cells in your sample express one isoform, the other isoform, or both isoforms. This capability is made possible by Milo's Single-Cell Western technology which surpasses other methods like flow cytometry