12S-HETE ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP3-07908

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Plasma, culture supernates (100 ul)

Sensitivity

146 pg/ml

Assay Range

195 - 50000 pg/ml
View all 12S-HETE ELISA Kits »

Product Specifications

Assay Type

Indirect ELISA

Kit Type

ELISA Kit (Colorimetric)

Specificity

Cross-reactivities for a number of related compounds:

12(R)-HETE: 2.5%
15-HETE: 0.3%
5(S)-HETE: 0.2%
8,15-diHETE: 0.1%
5,15-diHETE: 0.1%
PGE2: 0.1%
PGF2alpha: 0.1%
PGD2: 0.1%
6-keto-PGF1alpha: 0.1%
Thromboxane B2: 0.1%
Arachidonic Acid: 0.1%
Leukotriene B4: 0.1%
Leukotriene C4: 0.1%
Leukotriene D4: 0.1%
Leukotriene E4: 0.1%
8-HETE: 0.1%
9-HETE: 0.1%
11-HETE: 0.1%

Description

Store all components at 4C, except standard and conjugate at -20C.

Precision

Intra-Assay Precision (Precision within an assay) CV%: 15%

Inter-Assay Precision (Precision between assays) CV%: 21%

Recovery for 12S-HETE ELISA Kit (Colorimetric)

Recovery

Culture Media: 94%
Sodium heparin plasma 104

Scientific Data Images for 12S-HETE ELISA Kit (Colorimetric)

Non-species specific 12S-HETE ELISA Kit (Colorimetric) [NBP3-07908] - Standard curve

Kit Contents for 12S-HETE ELISA Kit (Colorimetric)

  • 12(S)-HETE Antibody
  • 12(S)-HETE Assay Layout Sheet
  • 12(S)-HETE Conjugate
  • 12(S)-HETE Standard
  • Assay Buffer Concentrate
  • Goat anti-Rabbit IgG Microtiter Plate
  • Plate Sealer
  • pNpp Substrate
  • Stop Solution
  • Wash Buffer Concentrate

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: 12S-HETE

Alternate Names

(5Z,8Z,10E,12S,14Z)-12-hydroxyicosa-5,8,10,14-tetraenoic acid, 12(S)-HETE, 12(S)-Hydroxyeicosatetraenoic acid, 12-Hete, 12-Hydroxyeicosatetraenoic acid, 12S-HETE, 54397-83-0, CHEBI:34146

Additional 12S-HETE Products

Product Documents for 12S-HETE ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for 12S-HETE ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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