14-3-3 gamma Antibody (S01-4C7)
Novus Biologicals | Catalog # NBP3-20083
Recombinant Monoclonal Antibody
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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # S01-4C7 expressed in HEK293
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Product Specifications
Immunogen
A synthetic peptide of human 14-3-3 gamma (Uniprot # P61981)
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for 14-3-3 gamma Antibody (S01-4C7)
Western Blot: 14-3-3 gamma Antibody (S01-4C7) [NBP3-20083]
Western Blot: 14-3-3 gamma Antibody (S01-4C7) [NBP3-20083] - Western blot detection of 14-3-3 gamma in HL-60, U251 cell lysates using NBP3-20083 (1:1000 diluted). Predicted band size: 28kDa. Observed band size: 28kDa.Applications for 14-3-3 gamma Antibody (S01-4C7)
Application
Recommended Usage
Western Blot
1/1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
Preservative
0.01% Sodium Azide
Concentration
0.3 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: 14-3-3 gamma
Signal-induced phosphorylation has the ability to change protein function, however phosphorylation is not always enough to change a protein's function. 14-3-3 gamma has the ability to bind a large number of target proteins thereby affecting the proteins' activity and/or function.
14-3-3 gamma is highly expressed in brain, heart and skeletal muscle, where it is induced by growth factors in human vascular smooth muscle cells. This suggests that 14-3-3 gamma may play a role in muscle tissue function.
Alternate Names
1433 gamma, YWHAG
Gene Symbol
YWHAG
Additional 14-3-3 gamma Products
Product Documents for 14-3-3 gamma Antibody (S01-4C7)
Certificate of Analysis
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Product Specific Notices for 14-3-3 gamma Antibody (S01-4C7)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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