2-Deoxy-D-glucose

Tocris Bioscience | Catalog # 4515

Non-metabolizable glucose analog; inhibits glucose metabolism
Tocris Bioscience

Key Product Details

Description

Non-metabolizable glucose analog; inhibits glucose metabolism
View all Mitochondrial Hexokinase Substrates »

Product Description

2-Deoxy-D-glucose is a non-metabolizable glucose analog. Inhibits phosphorylation of glucose by hexokinase; causes depletion of cellular ATP. Also inhibits phosphoglucose isomerase (PGI) competitively. Causes cell cycle inhibition and cell death in in vitro models of hypoxia; blocks tumor cell growth in animal models. Also shown to induce the unfolded protein response (UPR). 2-Deoxy-D-glucose disrupts N-glycan expression on the surface of pancreatic adenocarcinoma cells. 2-Deoxy-D-glucose enhances chimeric antigen receptor T cell activity in different xenograft mouse models of pancreatic adenocarcinoma cells.

Product Specifications for 2-Deoxy-D-glucose

Molecular Weight

164.16

Formula

C6H12O5

Storage

Store at +4°C

Chemical Name

2-Deoxy-D-arabino-hexose

CAS Number

154-17-6

PubChem ID

439268

InChI Key

PMMURAAUARKVCB-CERMHHMHSA-N

SMILES

OC1C[C@@H](O)[C@H](O)[C@@H](CO)O1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 16.42 100
DMSO 16.42 100

Preparing Stock Solutions for 2-Deoxy-D-glucose

The following data is based on the product molecular weight 164.16.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 6.09 mL 30.46 mL 60.92 mL
5 mM 1.22 mL 6.09 mL 12.18 mL
10 mM 0.61 mL 3.05 mL 6.09 mL
50 mM 0.12 mL 0.61 mL 1.22 mL

Calculators

Calculate the mass, volume, or concentration required for a solution.

=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Calculate the dilution required to prepare a stock solution.

x
=
x

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background References

References are publications that support the biological activity of the product. See our Citations tab to view 23 publications citing the usage of this product.

Product Documents for 2-Deoxy-D-glucose

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for 2-Deoxy-D-glucose

For research use only

Citations for 2-Deoxy-D-glucose

Customer Reviews for 2-Deoxy-D-glucose (4)

4.8 out of 5
4 Customer Ratings
5 Stars
75%
4 Stars
25%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used 2-Deoxy-D-glucose?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 4 of 4 reviews Showing All
Filter By:
Clear Filters X
  • 2-DG test for lactylation
    Name: Anonymous
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: 293T
    Verified Customer | Posted 02/17/2025
    2-DG at 10 mM was treated 293t cells for 24 hours
    2-Deoxy-D-glucose 4515
  • Good quality for in vitro study
    Name: Anonymous
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: MDA-MB-231
    Verified Customer | Posted 03/07/2020
    2-DG (10mM) was used to inhibit the glycolytic pathway in MDA-MB-231 cell lines.
    2-Deoxy-D-glucose 4515
  • Blockade of glycolysis at 5 mM enhances lipid peroxidation
    Name: Anonymous
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: human cancer lines
    Verified Customer | Posted 02/27/2020
    Dissolved in PBS to 500 mM. Suggest ordering a large size as it goes by quickly at 5mM.
    Used to inhibit glycolysis and influence activity of a lipid peroxidation enzyme. Good activity was observed at 5 mM with the majority of cell lines surviving. We found a few cancer cell lines where 5 uM was cytotoxic
    2-Deoxy-D-glucose 4515
  • Glycolysis block
    Name: Anonymous
    Species: Mouse
    Assay Type: Ex Vivo
    Cell Line/Tissue: acute brain slice
    Verified Customer | Posted 01/31/2018
    Used in brain slices to block glycolysis. Effect observed on cells within minutes of bulk application, as expected.

There are no reviews that match your criteria.

Showing  1 - 4 of 4 reviews Showing All