3-Azido-7-hydroxycoumarin
Tocris Bioscience | Catalog # 7664
Key Product Details
Description
Wavelength
Product Description
Key information:3-Azido-7-hydroxycoumarin is a water-soluble fluorogenic fluorescent dye. Supplied with an azide reactive handle for click chemistry. It emits intense fluorescence only after 'click' reaction when it forms triazoles at the site of alkyne modified biomolecules. Suitable for live-cell imaging.
Application: Fluorescence microscopy. Can be used to label DNA in proliferating cells following 5-Ethynyl-2'-deoxyuridine (Cat.No. 7207) incorporation. Cell permeable.
Properties and Photophysical Data: Excitation and emission maxima (λ) are 404 nm and 477 nm, respectively, after click reaction.
Optical Data
| Emission Color | Blue |
| λabs | 404 nm |
| λem | 477 nm |
| Application | Fluorescence Microscopy |
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Product Specifications for 3-Azido-7-hydroxycoumarin
Molecular Weight
Formula
Storage
Purity
Chemical Name
CAS Number
PubChem ID
InChI Key
SMILES
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMF | 20.32 | 100 | |
| DMSO | 20.32 | 100 |
Preparing Stock Solutions for 3-Azido-7-hydroxycoumarin
The following data is based on the product molecular weight 203.15.
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 4.92 mL | 24.61 mL | 49.22 mL |
| 5 mM | 0.98 mL | 4.92 mL | 9.84 mL |
| 10 mM | 0.49 mL | 2.46 mL | 4.92 mL |
| 50 mM | 0.10 mL | 0.49 mL | 0.98 mL |
Calculators
Background References
References are publications that support the biological activity of the product.
- Washington-Hughes In vivo virus-based macrofluorogenic probes target azide-labeled surface glycans in MCF-7 breast cancer cells. Mol.Pharm. 2013 PMID: 22998503
- Silini CM from intact hAM: an easily obtained product with relevant implications for translation in regenerative medicine. Stem Cell Res.Ther. 2021 PMID: 34641958
- Bobrin Water-borne nanocoating for rapid inactivation of SARS-CoV-2 and other viruses. ACS Nano 2021 PMID: 34423970
- Liu CLICK-17, a DNA enzyme that harnesses ultra-low concentrations of either Cu+ or Cu2+ to catalyze the azide-alkyne 'click' reaction in water. Nucleic Acids Res 2020 PMID: 32520335
- Li Fluorogenic "click" reaction for labeling and detection of DNA in proliferating cells. Biotechniques 2010 PMID: 20615206
- Sivakumar A fluorogenic 1,3-dipolar cycloaddition reaction of 3-azidocoumarins and acetylenes. Org.Lett. 2004 PMID: 15548086
Product Documents for 3-Azido-7-hydroxycoumarin
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for 3-Azido-7-hydroxycoumarin
For research use only
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