5-Ethynyl-2'-deoxyuridine

Key Product Details

Product Description

5-Ethynyl-2'-deoxyuridine is a thymidine analog for labeling DNA. Can be linked to an azide fluorophore, such as 3-Azido-7-hydroxycoumarin (Cat. No. 7664) through click chemistry and used to detect DNA synthesis in proliferating cells in vivo and in vitro. Detects very low levels of cell proliferation in tissues with low cellular turnover. 5-Ethynyl-2'-deoxyuridine suppresses cell proliferation in osteosarcoma and glioblastoma cell lines, induces a 'futile' DNA repair cycle and induces DNA strand breaks and apoptosis at a higher rate than Temozolomide (Cat. No. 2706).

Product Specifications for 5-Ethynyl-2'-deoxyuridine

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Preparing Stock Solutions for 5-Ethynyl-2'-deoxyuridine

The following data is based on the product molecular weight 252.23.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

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Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.96 mL	19.82 mL	39.65 mL
5 mM	0.79 mL	3.96 mL	7.93 mL
10 mM	0.40 mL	1.98 mL	3.96 mL
50 mM	0.08 mL	0.40 mL	0.79 mL

Calculators

Background References

References are publications that support the biological activity of the product.

• Wang Nucleotide excision repair removes thymidine analog 5-ethynyl-2'-deoxyuridine from the mammalian genome. Proc.Natl.Acad.Sci.U.S.A. 2022 PMID: 35994676
• Salic & Mitchison A chemical method for fast and sensitive detection of DNA synthesis in vivo. Proc.Natl.Acad.Sci.U.S.A. 2008 PMID: 18272492

Product Documents for 5-Ethynyl-2'-deoxyuridine