3-Deazaneplanocin A hydrochloride

Key Product Details

Product Description

3-Deazaneplanocin A (DZNep) hydrochloride is a histone methyltransferase inhibitor; decreases global histone methylation. 3-Deazaneplanocin A hydrochloride inhibits EZH2 histone methyltransferase and s-adenosylhomocysteine (SAH) hydrolase activity. Blocks trimethylation of lysine 27 on histone H3 and lysine 20 on histone H4 in vitro. 3-Deazaneplanocin A hydrochloride induces apoptosis in multiple cancer cell lines and has no apoptotic effect on normal cells. Enhances Oct4 expression in chemically induced pluripotent stem cells (CiPSCs). Combined with other reagents, 3-Deazaneplanocin A hydrochloride enables reprogramming of mouse embryonic fibroblasts (MEFs) to chemically-induced pluripotent stem cells (CiPSCs) (see our protocol below).

DZNep synthesized to Ancillary Material Grade also available.

For more information about how 3-Deazaneplanocin A hydrochloride may be used, see our protocol: Highly Efficient Generation of CiPSCs from MEFs

Product Specifications for 3-Deazaneplanocin A hydrochloride

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Preparing Stock Solutions for 3-Deazaneplanocin A hydrochloride

The following data is based on the product molecular weight 298.73.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

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Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.1 mM	33.48 mL	167.38 mL	334.75 mL
0.5 mM	6.70 mL	33.48 mL	66.95 mL
1 mM	3.35 mL	16.74 mL	33.48 mL
5 mM	0.67 mL	3.35 mL	6.70 mL

Calculators

Background References

References are publications that support the biological activity of the product. See our Citations tab to view 10 publications citing the usage of this product.

• Hou Pluripotent stem cells induced from mouse somatic cells by small-molecule compounds. Science 2013 PMID: 23868920
• Miranda DZNep is a global histone methylation inhibitor that reactivates developmental genes not silenced by DNA methylation. Mol.Cancer Ther. 2009 PMID: 19509260
• Tseng Synthesis of 3-deazaneplanocin A, a powerful inhibitor of S-adenosylhomocysteine hydrolase with potent and selective in vitro and in vivo antiviral activities. J.Med.Chem. 1989 PMID: 2544721
• Tan Pharmacologic disruption of Polycomb-repressive complex 2-mediated gene repression selectively induces apoptosis in cancer cells. Genes Dev. 2007 PMID: 17437993

Product Documents for 3-Deazaneplanocin A hydrochloride