4-Thiouridine

Key Product Details

Product Description

4-Thiouridine is a photoreactive uridine analog for RNA sequencing (RNA seq) and thiol(SH)-linked alkylation for the metabolic sequencing of RNA (SLAM seq). Used as a crosslinker to identify RNA binding proteins. Is incorporated into newly transcribed RNA and can be used to track RNA from synthesis to degradation.

Product Specifications for 4-Thiouridine

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Preparing Stock Solutions for 4-Thiouridine

The following data is based on the product molecular weight 260.26.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

3 2 1 Recalculate

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.2 mM	19.21 mL	96.06 mL	192.12 mL
1 mM	3.84 mL	19.21 mL	38.42 mL
2 mM	1.92 mL	9.61 mL	19.21 mL
10 mM	0.38 mL	1.92 mL	3.84 mL

Calculators

Background References

References are publications that support the biological activity of the product. See our Citations tab to view 12 publications citing the usage of this product.

• Herzog Thiol-linked alkylation of RNA to assess expression dynamics. Nat.Methods 2017 PMID: 28945705
• Schofield TimeLapse-seq: adding a temporal dimension to RNA sequencing through nucleoside recoding. Nat.Methods 2018 PMID: 29355846
• Garibaldi Isolation of newly transcribed RNA using the metabolic label 4-thiouridine. Methods Mol.Biol. 2017 PMID: 28766297

Product Documents for 4-Thiouridine