5-HT4 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-99479

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all 5-HT4 Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human 5-HT4.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for 5-HT4 Antibody - BSA Free

Western Blot: 5-HT4 Antibody [NBP2-99479]

Western Blot: 5-HT4 Antibody [NBP2-99479]

Western Blot: 5-HT4 Antibody [NBP2-99479] - Anti-5-HT4 rabbit polyclonal antibody at 1:500 dilution. Lane A: SW480 Whole Cell Lysate. Lane B: HeLa Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 43 kDa. Observed band size: 44 kDa
Immunoprecipitation: 5-HT4 Antibody [NBP2-99479]

Immunoprecipitation: 5-HT4 Antibody [NBP2-99479]

Immunoprecipitation: 5-HT4 Antibody [NBP2-99479] - 5-HT4 was immunoprecipitated using: Lane A: 0.5 mg HeLa Whole Cell Lysate. 4 ul anti-5-HT4 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein A/G. Primary antibody: Anti-5-HT4 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 43 kDa. Observed band size: 43 kDa

Applications for 5-HT4 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

5-10 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Format

BSA Free

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: 5-HT4

The 5-HT4 receptor, a member of the Serotonin Receptor subfamily, has been shown to affect the sensory and motor functions of the gut. Some of the most promising emerging therapies in Irritable Bowel Syndrome revolve around targeted pharmacotherapeutic modulation of serotonin receptors (ie, 5-HT3 and 5-HT4 subtypes). At least 9 isoforms (5-HT4A-5-HT4G, and 5-HT4n) have been described, which differ in the length and composition of their intracellular C termini after a common splicing site (L358). A ninth isoform (5-HT4H) results in the insertion of 14 amino acids into the second intracellular loop. 5-HT4 receptor expression has been documented primarily in the brain, and in colon, heart, and vessel. An EST has been isolated from a human cancerous nerve library.

Long Name

5-Hydroxytryptamine Receptor 4

Alternate Names

5HT4, HTR4

Gene Symbol

HTR4

Additional 5-HT4 Products

Product Documents for 5-HT4 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for 5-HT4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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