5X COOMASSIEnano Concentrate Protein Staining Solution

Novus Biologicals | Catalog # NBP3-33179

Novus Biologicals
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Key Product Details

Applications

Western Blot

Product Summary for 5X COOMASSIEnano Concentrate Protein Staining Solution

5X COOMASSIE nano, innovatively enhanced by proprietary nano-technology, is a concentrated, easy-to-reconstitute protein staining solution for SDS-PAGE gels. Coomassie blue protein staining is widely used in gel electrophoresis techniques for visualizing protein bands. This next generation Coomassie dye is formulated for quick protein detection and higher sensitivity. Additionally, 5X COOMASSIE nano eliminates the need for traditional destaining and wash steps. In the absence of hazardous substances such as methanol and acetic acid, 5X COOMASSIE nano is considered safe and environmentally friendly by reducing carbon footprint by as much as 80%. Besides western blot, this Coomassie protein stain is also compatible with mass spectrophotometry.

Key Features:

  • Quick Staining - Achieve vibrant blue staining results in minutes

  • No Destaining Required

  • No Washing Step Needed

  • Non-Hazardous Material - Ensures a safe and environmentally friendly staining experience

  • High Sensitivity
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Product Specifications

Application Notes

There is no risk of excessive staining. Instead, superior visibility for each band is achieved when the gel is submerged overnight. Carefully mix COOMASSIE nano up and down few times before use. If the gel is thicker than mini-gel (1mm), it will take longer to incubate. The staining solution may contain blue clumps. This is normal and easily dissolved in distilled water

Scientific Data Images for 5X COOMASSIEnano Concentrate Protein Staining Solution

5X COOMASSIEnano Concentrate Protein Staining Solution

Western Blot: 5X COOMASSIEnano Concentrate Protein Staining Solution [NBP3-33179] -

Western Blot: 5X COOMASSIEnano Concentrate Protein Staining Solution [NBP3-33179] - COOMASSIEnano Protein Staining Solution practices the fast-signal representing at 5 min. Prestained protein ladder, unstain protein ladder, and BSA were prepared and applied in electrophoresis. After running SDS-PAGE (4-20% Novex Gel), please remove the gel from the cassette then proceed to submerge the gel in proper amount of COOMASSIEnano dye, enough to cover the gel. Lightly agitate the staining box for 5 minutes to over night at room temperature.
5X COOMASSIEnano Concentrate Protein Staining Solution

Western Blot: 5X COOMASSIEnano Concentrate Protein Staining Solution [NBP3-33179] -

Western Blot: 5X COOMASSIEnano Concentrate Protein Staining Solution [NBP3-33179] - COOMASSIEnano Protein Staining Solution practices the fast-signal representing at 5 min. Prestained protein ladder, unstain protein ladder, and BSA were prepared and applied in electrophoresis. After running SDS-PAGE (4-20% Novex Gel), please remove the gel from the cassette then proceed to submerge the gel in proper amount of COOMASSIEnano dye, enough to cover the gel. Lightly agitate the staining box for 5 minutes to over night at room temperature.

Formulation, Preparation, and Storage

Preparation Method

Prepare a 5L bottle. Unpack and pour the liquid content into the bottle.Rinse residual reagent in the original bottle with deionized water. Add deionized water until the volume reaches 5L.

Concentration

Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at room temperature.

Background: 5X COOMASSIEnano Concentrate Protein Staining Solution

Alternate Names

COOMASSIE, Coomassie brilliant blue

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Product Documents for 5X COOMASSIEnano Concentrate Protein Staining Solution

Certificate of Analysis

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Product Specific Notices for 5X COOMASSIEnano Concentrate Protein Staining Solution

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

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