Chemical Name: 2,7-Diamino-N1,N9-bis(6,13-diisopropyl-2,5,9-trimethyl-1,4,7,11,14-pentaoxohexadecahydro-1H-pyrrolo[2,1-i]oxa[4,7,10,13]tetraazacyclohexadecin-10-yl)-4,6-dimethyl-3-oxo-3H-phenoxazine-1,9-dicarboxamide
Biological Activity7-Aminoactinomycin D is a fluorescent DNA stain. G-C base-specific DNA intercalator that forms stable complexes with DNA. Suitable for use in flow cytometry. Detects apoptotic cells. May be used in combination with FITC (Cat. No. 5440) or phycoerythrin (PE). Fluorescent analogue of actinomycin D (Cat. No. 1229). Excitation/emission maxima (λ) = 488/655 nm.
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
7-Amino-actinomycin D as a specific fluorophore for DNA content analysis by laser flow cytometry.
Zelenin et al.
Dead cell discrimination with 7-amino-actinomycin D in combination with dual color immunofluorescence in single laser flow cytometry.
Schmid et al.
The use of 7-amino actinomycin D in identifying apoptosis: Simplicity of use and broad spectrum of application compared with other techniques.
Philpott et al.
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