7-Aminoactinomycin D

Tocris Bioscience | Catalog # 7121

Red-fluorescent DNA stain, membrane impermeant to live cells. Used for: apoptosis detection, cell viability staining. Application: flow cytometry, fluorescence microscopy
Tocris Bioscience

Key Product Details

Description

Red-fluorescent DNA stain, membrane impermeant to live cells. Used for: apoptosis detection, cell viability staining. Application: flow cytometry, fluorescence microscopy

Wavelength

Excitation = 549 nm, Emission = 655 nm
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Product Description

Key information:

7-aminoactinomycin D (7-AAD) is a red-fluorescent DNA stain. It is membrane impermeant to live cells but penetrates dead or damaged cells.

Used for: cell cycle studies, selective detection of dead cells by flow cytometry, chromosome banding analysis, cell sorting, and apoptosis quantification.

Application: fluorescence microscopy and flow cytometry.

Properties and Photophysical Data: G-C base-specific DNA intercalator that forms stable complexes with DNA. May be used in combination with FITC (Cat. No. 5440) or phycoerythrin (PE). Fluorescent analogue of actinomycin D (Cat. No. 1229). Can be excited using 488 nm (blue), 532 nm (green), and 561 nm (yellow-green) laser lines. Suitable for multicolor fluorescence microscopy experiments. Excitation and emission maxima (λ) are 549 nm and 655 nm, respectively.

Optical Data

7-Aminoactinomycin D Dye Spectra
λabs 549 nm
λem 655 nm
Application Fluorescence Microscopy, Flow Cytometry

Spectra Viewer

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Product Specifications for 7-Aminoactinomycin D

Molecular Weight

1270.45

Formula

C62H87N13O16

Storage

Store at -20°C

Purity

≥97% (HPLC)

Chemical Name

2,7-Diamino-N1,N9-bis(6,13-diisopropyl-2,5,9-trimethyl-1,4,7,11,14-pentaoxohexadecahydro-1H-pyrrolo[2,1-i][1]oxa[4,7,10,13]tetraazacyclohexadecin-10-yl)-4,6-dimethyl-3-oxo-3H-phenoxazine-1,9-dicarboxamide

CAS Number

7240-37-1

PubChem ID

14924508

InChI Key

YXHLJMWYDTXDHS-UHFFFAOYSA-N

SMILES

NC1=C(C(NC(C(C)OC(C(C(C)C)N(C(CN(C)C2=O)=O)C)=O)C(NC(C(C)C)C(N3C2CCC3)=O)=O)=O)C4=NC5=C(C(NC(C(NC(C(C)C)C6=O)=O)C(C)OC(C(C(C)C)N(C(CN(C)C(C7N6CCC7)=O)=O)C)=O)=O)C=C(N)C(C)=C5OC4=C(C)C1=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 2 2

Preparing Stock Solutions for 7-Aminoactinomycin D

The following data is based on the product molecular weight 1270.45.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.02 mM 39.36 mL 196.78 mL 393.56 mL
0.1 mM 7.87 mL 39.36 mL 78.71 mL
0.2 mM 3.94 mL 19.68 mL 39.36 mL
1 mM 0.79 mL 3.94 mL 7.87 mL

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Product Documents for 7-Aminoactinomycin D

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for 7-Aminoactinomycin D

For research use only

Citations for 7-Aminoactinomycin D

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