Catalog Number: 1229
Chemical Name: 2-Amino-(N,N)-1-bis(hexadecahydro-6,13-diisopropyl-2,5,9-trimethyl-1,4,7,11,14-pentaoxo-1H-pyrrolo[2,1]-[1,4,7,10,13] oxatetraazacyclohexadecin-10-yl)-4,6-dimethyl-3-oxo-3H-phenoxazine-1,9-dicarboxamide
Biological Activity
Anti-neoplastic antibiotic. Inhibits RNA polymerase and is a potent inducer of apoptosis.
Technical Data
  • M.Wt:
    1255.43
  • Formula:
    C62H86N12O16
  • Solubility:
    Soluble to 50 mM in DMSO
  • Purity:
    >98%
  • Storage:
    Desiccate at +4°C
  • CAS No:
    50-76-0
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis. All Tocris products are intended for laboratory research use only.
Background References
  1. A kinetic study on the mechanism of inhibition of RNA synthesis catalyzed by DNA-dependent RNA polymerase. Differences in inhibition by ethidium bromide, 3,8-diamino-6-ethylphenanthridinium bromide and actinomycin D.
    Aktipis et al.
    Biochim.Biophys.Acta, 1981;655:278
  2. Apoptosis induced by actinomycin D, camptothecin or aphidicolin can occur in all phases of the cell cycle.
    Glynn et al.
    Biochem.Soc.Trans., 1992;20:84S
  3. The mechanism of actinomycin D-mediated inhibition of HIV-1 reverse transcription.
    Jeeninga et al.
    Nucleic Acids Res., 1998;26:5472
Citations:

The citations listed below are publications that use Tocris products. Selected citations for Actinomycin D include:

13 Citations: Showing 1 - 10
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  1. Acute Stress Suppresses Synaptic Inhibition and Increases Anxiety via Endocannabinoid Release in the Basolateral Amygdala.
    Authors: Di Et al.
    J.Neurosci. 2016;36:8461
  2. Integrin-FAK signaling rapidly and potently promotes mitochondrial function through STAT3.
    Authors: Visavadiya
    Cell Communication and Signaling 2016;14:32
  3. SynGAP regulates protein synthesis and homeostatic synaptic plasticity in developing cortical networks.
    Authors: Wang Et al.
    PLoS One 2014;8:e83941
  4. Microplate-based screening for small molecule inhibitors of neuropilin-2/vascular endothelial growth factor-C interactions.
    Authors: Parker and Kooi
    J Biol Chem 2014;453:42525
  5. The anticancer agent di-2-pyridylketone 4,4-dimethyl-3-thiosemicarbazone (Dp44mT) overcomes prosurvival autophagy by two mechanisms: persistent induction of autophagosome synthesis and impairment of lysosomal integrity.
    Authors: Gutierrez Et al.
    J Biol Chem 2014;289:33568
  6. Downregulation of BRCA1 protein in BCR-ABL1 leukemia cells depends on stress-triggered TIAR-mediated suppression of translation.
    Authors: Podszywalow-Bartnicka Et al.
    Cardiovasc Res 2014;13:3727
  7. Loss of neuron-astroglial interaction rapidly induces protective CNTF expression after stroke in mice.
    Authors: Kang Et al.
    J Neurosci 2012;32:9277
  8. Endothelial nitric oxide synthase enhancer reduces oxidative stress and restores endothelial function in db/db mice.
    Authors: Cheang Et al.
    Biochem Biophys Res Commun 2011;92:267
  9. MEF-2 regulates activity-dependent spine loss in striatopallidal medium spiny neurons.
    Authors: Tian Et al.
    Mol Cell Neurosci 2010;44:94
  10. Posttranscriptional regulation of angiotensin II type 1 receptor expression by glyceraldehyde 3-phosphate dehydrogenase.
    Authors: Backlund Et al.
    Nucleic Acids Res 2009;37:2346
  11. Cyclooxygenase-2-derived prostaglandin F2alpha mediates endothelium-dependent contractions in the aortae of hamsters with increased impact during aging.
    Authors: Wong Et al.
    Circ Res 2009;104:228
  12. p100 increases AT1R expression through interaction with AT1R 3'-UTR.
    Authors: Paukku Et al.
    Nucleic Acids Res 2008;36:4474
  13. Heat shock protein 60 or 70 activates nitric-oxide synthase (NOS) I- and inhibits NOS II-associated signaling and depresses the mitochondrial apoptotic cascade during brain stem death.
    Authors: Chan Et al.
    Cell Cycle 2007;282:4585
Expand to show all 13 Citations

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