Actinomycin D

Tocris Bioscience | Catalog # 1229

Inhibits RNA polymerase
Tocris Bioscience

Key Product Details

Description

Inhibits RNA polymerase
View all RNA/DNA Polymerase Inhibitors »

Product Description

Actinomycin D is an anti-neoplastic antibiotic. Inhibits RNA polymerase and is a potent inducer of apoptosis.

Product Specifications for Actinomycin D

Molecular Weight

1255.43

Formula

C62H86N12O16

Storage

Store at -20°C

Purity

≥95% (HPLC)

Chemical Name

2-Amino-(N,N)-1-bis(hexadecahydro-6,13-diisopropyl-2,5,9-trimethyl-1,4,7,11,14-pentaoxo-1H-pyrrolo[2,1]-[1,4,7,10,13] oxatetraazacyclohexadecin-10-yl)-4,6-dimethyl-3-oxo-3H-phenoxazine-1,9-dicarboxamide

CAS Number

50-76-0

PubChem ID

2019

InChI Key

RJURFGZVJUQBHK-DCUCITLNSA-N

SMILES

[H][C@@]12CCCN1C([C@H](C(C)C)NC([C@H]([C@H](OC([C@@H](N(C(CN(C2=O)C)=O)C)C(C)C)=O)C)NC(C3=C4N=C5C(OC4=C(C=C3)C)=C(C(C(N)=C5C(N[C@H]6[C@H](OC([C@@H](N(C(CN(C([C@@]7(CCCN7C([C@H](C(C)C)NC6=O)=O)[H])=O)C)=O)C)C(C)C)=O)C)=O)=O)C)=O)=O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 62.77 50

Preparing Stock Solutions for Actinomycin D

The following data is based on the product molecular weight 1255.43.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.5 mM 1.59 mL 7.97 mL 15.93 mL
2.5 mM 0.32 mL 1.59 mL 3.19 mL
5 mM 0.16 mL 0.80 mL 1.59 mL
25 mM 0.03 mL 0.16 mL 0.32 mL

Calculators

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 115 publications citing the usage of this product.

Product Documents for Actinomycin D

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Actinomycin D

For research use only

⚠ WARNING: This product can expose you to chemicals including Actinomycin D, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov

Related Research Areas

Epigenetics

Customer Reviews for Actinomycin D (2)

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  • Mitochondrial bioenergetics measurements by reagent screen
    Name: Anonymous
    Species: Mouse
    Assay Type: In Vitro
    Cell Line/Tissue: mouse brain endothelioma cell line
    Verified Customer | Posted 03/15/2020
    0.3 μg/ml
    Actinomycin D 1229
  • In vivo assay
    Name: Anonymous
    Species: Human
    Assay Type: In Vitro
    Verified Customer | Posted 01/07/2018
    I used in my experiment for muHL6b cells with 0.2 μg/ml actinomycin D final concentration.

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