A-431 Whole Cell Lysate

Novus Biologicals | Catalog # NB800-PC12

Novus Biologicals
100% Guarantee

Key Product Details

Species

Human

Applications

Western Blot

Product Specifications

Application Notes

Ready-to-use A431 Whole Cell Lysate are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOuT dissociating agents. Heat lysate - 95C for 5 minutes and rapidly cool. If dissociating conditions are desired, add reducing agent prior - heating. The recommended loading volume per lane is 10-20 ul depending on the size format of your gel.

Specificity

A431 whole cell extract lysate

Type

Cell

Tissue

Skin

Subcellular Fraction

Whole

Scientific Data Images for A-431 Whole Cell Lysate

Western Blot: A-431 Whole Cell Lysate [NB800-PC12]

Western Blot: A-431 Whole Cell Lysate [NB800-PC12]

Western Blot: A-431 Whole Cell Lysate [NB800-PC12] - Coommassie stained SDS-PAGE of 40 ug of Human Derived A431 Whole Cell Lysate separated in a 4-20% gradient gel under non-reducing conditions.
Western Blot: A-431 Whole Cell Lysate [NB800-PC12]

Western Blot: A-431 Whole Cell Lysate [NB800-PC12]

Western Blot: A-431 Whole Cell Lysate [NB800-PC12] - Coommassie stain of 0.02 ml of NB 800-PC12 separated in a 4-20% gradient gel under non-reducing conditions (lane 1). MW standard is shown in lane 2.

Formulation, Preparation, and Storage

Formulation

1X SDS-PAGE Sample Buffer [0.05 mM Tris HCl, 2% SDS, 10% Glycerol, 0.1% bromophenol blue, pH 6.8]

Concentration

1 mg/ml

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -80C. Avoid freeze-thaw cycles.

Background: A-431

This ready-to-use whole cell lysate is derived from a cell line or tissue using highly refined extraction protocols to ensure exceptionally high quality, protein integrity and lot-to-lot reproducibility. All extracts are tested by SDS-PAGE using 4-20% gradient gels and immunoblot analysis using antibodies to key cell signaling components to confirm the presence of both high molecular weight and low molecular weight proteins.

Additional A-431 Products

Product Documents for A-431 Whole Cell Lysate

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for A-431 Whole Cell Lysate

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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