AATF [p Ser477] Antibody - Azide Free

Novus Biologicals | Catalog # NBP2-29517

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Primate (100%), Rat (100%), Sheep (100%), Xenopus (100%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide Free
View all AATF Primary Antibodies »

Product Specifications

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser477 conjugated to KLH. Accession # Q9NY61

Modification

p Ser477

Specificity

Specific for endogenous levels of the ~66 kDa AATF protein phosphorylated at Ser477. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

66 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for AATF [p Ser477] Antibody - Azide Free

Western Blot: AATF [p Ser477] Antibody [NBP2-29517]

Western Blot: AATF [p Ser477] Antibody [NBP2-29517]

Western Blot: AATF [p Ser477] Antibody [NBP2-29517] - Western blot of HeLa lysate showing specific immunolabeling of the ~66k Che-1 protein phosphorylated at Ser477. The phosphospecificity is shown in the second lane where immunoreactivity is blocked by preadsorption with the phospho-peptide (Peptide) used as antigen but not by the dephosphopeptide (not shown).

Applications for AATF [p Ser477] Antibody - Azide Free

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Format

Azide Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: AATF

Che-1, also known as AATF (apoptosis-antagonizing transcription factor), is a RNA polymerase II-binding protein involved in regulating the transcription factor E2F and promoting cell cycle progression (Burgdorf et al., 2004). It has been suggested that Che-1 may act as a neuroprotective factor against Abeta-induced apoptosis by suppressing the production of reactive oxidative species (Xie et al., 2004). The checkpoint kinase Chk2 has been shown to phosphorylate Che-1 at Ser477 contributing to the maintenance of the G2/M checkpoint induced by DNA damage (Bruno et al., 2006).

Alternate Names

apoptosis antagonizing transcription factor, Apoptosis-antagonizing transcription factor, CHE-1, CHE1Rb-binding protein Che-1, DEDprotein AATF

Entrez Gene IDs

26574 (Human)

Gene Symbol

AATF

UniProt

Q9NY61 (Human)

Additional AATF Products

Product Documents for AATF [p Ser477] Antibody - Azide Free

Certificate of Analysis

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Product Specific Notices for AATF [p Ser477] Antibody - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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