ACE/CD143 Antibody (1C9J3)

Novus Biologicals | Catalog # NBP3-15373

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Mouse, Rat

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1C9J3 expressed in HEK293
View all ACE/CD143 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1200-1306 of human ACE/CD143 (P12821). YFKPLLDWLRTENELHGEKLGWPQYNWTPNSARSEGPLPDSGRVSFLGLDLDAQQARVGQWLLLFLGIALLVATLGLSQRLFSIRHRSLHRHSHGPQFGSEVELRHS

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit ACE/CD143 Antibody (1C9J3) (NBP3-15373) is a recombinant monoclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for ACE/CD143 Antibody (1C9J3)

Western Blot: ACE/CD143 Antibody (1C9J3) [NBP3-15373]

Western Blot: ACE/CD143 Antibody (1C9J3) [NBP3-15373]

Western Blot: ACE/CD143 Antibody (1C9J3) [NBP3-15373] - Western blot analysis of extracts of various cell lines, using ACE/CD143 Rabbit mAb (NBP3-15373) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunoprecipitation: ACE/CD143 Antibody (1C9J3) [NBP3-15373]

Immunoprecipitation: ACE/CD143 Antibody (1C9J3) [NBP3-15373]

Immunoprecipitation: ACE/CD143 Antibody (1C9J3) [NBP3-15373] - Immunoprecipitation analysis of 600ug extracts of Mouse kidney cells using 3ug ACE/CD143 antibody (NBP3-15373). Western blot was performed from the immunoprecipitate using ACE/CD143 antibody (NBP3-15373) at a dilition of 1:1000.

Applications for ACE/CD143 Antibody (1C9J3)

Application
Recommended Usage

Immunoprecipitation

1:500 - 1:1000

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ACE/CD143

ACE converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilatator. ACE exists in two forms, a 170KD somatic form and a 90KD germinal form. The somatic form is expressed by endothelial cells (especially those of lung capillaries and arterioles), epithelial cells (especially in proximal renal tubules and small intestine), by some neuronal cells and variably by some macrophages and T lymphocytes. The germinal form is expressed by spermatozoa.

Long Name

Angiotensin I Converting Enzyme

Alternate Names

CD143, DCP1, Kininase II

Gene Symbol

ACE

Additional ACE/CD143 Products

Product Documents for ACE/CD143 Antibody (1C9J3)

Certificate of Analysis

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Product Specific Notices for ACE/CD143 Antibody (1C9J3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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