Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ACSL1 Primary Antibodies »

Product Specifications

Immunogen

Rabbit polyclonal ACSL1 antibody was raised against an 18 amino acid peptide near the center of human ACSL1. The immunogen is located within amino acids 240 - 290 of ACSL1. Amino Acid Squence: CDLGRANRRKPKPPAPEDL

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Guinea pig: (88%)

Specificity

At least three isoforms of ACSL1 are known to exist; this antibody will detect all three isoforms.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

45, 47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ACSL1 Antibody - BSA Free

Western Blot: ACSL1 AntibodyBSA Free [NBP2-82060]

Western Blot: ACSL1 AntibodyBSA Free [NBP2-82060]

Western Blot: ACSL1 Antibody [NBP2-82060] - Western blot analysis of ACSL1 in human lung tissue lysate with ACSL1 antibody at (A) 1 and (B) 2 ug/mL
ACSL1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: ACSL1 Antibody - BSA Free [NBP2-82060] -

Immunocytochemistry/ Immunofluorescence: ACSL1 Antibody - BSA Free [NBP2-82060] - Immunofluorescence of ACSL1 in human lung tissue with ACSL1 antibody at 20 ug/mL.

Applications for ACSL1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Western Blot

0.25 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ACSL1

Long-chain-fatty-acid--CoA ligase 1, or ACSL1, is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. These proteins play an important role in lipid biosynthesis and fatty acid degradation. Like other isozymes of this family, ALCS1 converts free long-chain fatty acids into fatty acyl-CoA esters. ACSL1 preferentially uses palmitoleate, oleate and linoleate, and altered expression of ACSL1 is thought to increase accumulation of triglycerides in the liver.

ALCS1 is strongly expressed in the heart, kindey, liver, skeletal muscle, and erythroid cells, and to a lesser extent in lung, brain, placenta and pancreas. This protein is predominantly expressed during the early stages of erythroid development, and expression is weak in reticulocytes and young erythrocytes.

Alternate Names

ACS1LACS 2, Acyl-CoA synthetase 1, acyl-CoA synthetase long-chain family member 1, EC 6.2.1, FACL1EC 6.2.1.3, fatty-acid-Coenzyme A ligase, long-chain 1, LACS 1, LACSlong-chain 2, lignoceroyl-CoA synthase, Long-chain acyl-CoA synthetase 1, Long-chain acyl-CoA synthetase 2, Long-chain fatty acid-CoA ligase 2, long-chain fatty-acid-coenzyme A ligase 1, long-chain-fatty-acid--CoA ligase 1, Palmitoyl-CoA ligase 1, Palmitoyl-CoA ligase 2, paltimoyl-CoA ligase 1

Gene Symbol

ACSL1

Additional ACSL1 Products

Product Documents for ACSL1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ACSL1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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