ACTR3 Antibody (8K4F2)

Novus Biologicals | Catalog # NBP3-16424

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 8K4F2 expressed in HEK293
View all ACTR3 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 319-418 of human ACTR3 (P61158). IVLSGGSTMFRDFGRRLQRDLKRTVDARLKLSEELSGGRLKPKPIDVQVITHHMQRYAVWFGGSMLASTPEFYQVCHTKKDYEEIGPSICRHNPVFGVMS

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ACTR3 Antibody (8K4F2)

Western Blot: ACTR3 Antibody (8K4F2) [NBP3-16424]

Western Blot: ACTR3 Antibody (8K4F2) [NBP3-16424]

Western Blot: ACTR3 Antibody (8K4F2) [NBP3-16424] - Western blot analysis of extracts of various cell lines, using ACTR3 Rabbit mAb (NBP3-16424) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Immunocytochemistry/ Immunofluorescence: ACTR3 Antibody (8K4F2) [NBP3-16424]

Immunocytochemistry/ Immunofluorescence: ACTR3 Antibody (8K4F2) [NBP3-16424]

Immunocytochemistry/Immunofluorescence: ACTR3 Antibody (8K4F2) [NBP3-16424] - Immunofluorescence analysis of C6 cells using ACTR3 Rabbit mAb (NBP3-16424) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ACTR3 Antibody (8K4F2)

Immunohistochemistry: ACTR3 Antibody (8K4F2) [NBP3-16424] -

Immunohistochemistry: ACTR3 Antibody (8K4F2) [NBP3-16424] - Immunohistochemistry analysis of ACTR3 in paraffin-embedded human tonsil tissue using ACTR3 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ACTR3 Antibody (8K4F2)

Immunocytochemistry/ Immunofluorescence: ACTR3 Antibody (8K4F2) [NBP3-16424] -

Immunocytochemistry/ Immunofluorescence: ACTR3 Antibody (8K4F2) [NBP3-16424] - Confocal imaging of U-2 OS cells using ACTR3 Rabbit mAb. The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
ACTR3 Antibody (8K4F2)

Immunohistochemistry: ACTR3 Antibody (8K4F2) [NBP3-16424] -

Immunohistochemistry: ACTR3 Antibody (8K4F2) [NBP3-16424] - Immunohistochemistry analysis of ACTR3 in paraffin-embedded mouse spleen tissue using ACTR3 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ACTR3 Antibody (8K4F2)

Immunohistochemistry: ACTR3 Antibody (8K4F2) [NBP3-16424] -

Immunohistochemistry: ACTR3 Antibody (8K4F2) [NBP3-16424] - Immunohistochemistry analysis of ACTR3 in paraffin-embedded rat brain tissue using ACTR3 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ACTR3 Antibody (8K4F2)

Immunohistochemistry: ACTR3 Antibody (8K4F2) [NBP3-16424] -

Immunohistochemistry: ACTR3 Antibody (8K4F2) [NBP3-16424] - Immunohistochemistry analysis of ACTR3 in paraffin-embedded human colon carcinoma tissue using ACTR3 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Applications for ACTR3 Antibody (8K4F2)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ACTR3

The specific function of this gene has not yet been determined; however, the protein it encodes is known to be a major constituent of the ARP2/3 complex. This complex is located at the cell surface and is essential to cell shape and motility through lamellipodial actin assembly and protrusion.

Long Name

Actin-related protein 3

Alternate Names

ARP3

Gene Symbol

ACTR3

Additional ACTR3 Products

Product Documents for ACTR3 Antibody (8K4F2)

Certificate of Analysis

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Product Specific Notices for ACTR3 Antibody (8K4F2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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