Human ADAMTS5 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-66444

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.47 ng/mL

Assay Range

0.78 - 50 ng/mL
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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Specificity

This kit recognizes Human ADAMTS5 in samples. No significant cross-reactivity or interference between Human ADAMTS5 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% 5.09%

Inter-Assay Precision (Precision between assays) CV% 5.09%

Recovery for Human ADAMTS5 ELISA Kit (Colorimetric)

Recovery

86-110%

Linearity

Human ADAMTS5 ELISA Kit (Colorimetric)

Scientific Data Images for Human ADAMTS5 ELISA Kit (Colorimetric)

Human ADAMTS5 ELISA Kit (Colorimetric)

ELISA: Human ADAMTS5 ELISA Kit (Colorimetric) [NBP2-66444] -

ELISA: Human ADAMTS5 ELISA Kit (Colorimetric) [NBP2-66444] - Standard Curve Reference

Kit Contents for Human ADAMTS5 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: ADAMTS5

ADAMTS5, also known as aggrecanase-2, is a Metallopeptidase M12B type protease that is responsible for degradation of the articular cartilage protein aggrecan. The protein contains a signal sequence, a prodomain with both a potential cysteine switch and a furin cleavage site, a metalloproteinase domain with a conserved zinc-binding motif and a 'met turn,' a disintegrin-like domain, and a spacer region between a thrombospondin type 1 motif and thrombospondin submotif. At least four transcript variants have been reported. ADAMTS5 has been implicated in inflammatory joint diseases. ADAMTS5 expression has been documented in a range of normal human tissues. ESTs have been isolated from human tissue libraries, including normal breast, embryo and heart and cancerous placenta and uterus.

Long Name

A Disintegrin-like and Metalloproteinase Domain with Thrombospondin Motifs 5

Alternate Names

ADAMTS11, ADMP-2, Aggrecanase 2

Gene Symbol

ADAMTS5

Additional ADAMTS5 Products

Product Documents for Human ADAMTS5 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Human ADAMTS5 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Related Research Areas

Metalloproteases and Regulators

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