Adenine Nucleotide Translocator 2 Recombinant Protein Antigen

Novus Biologicals | Catalog # NBP2-56762PEP

Novus Biologicals
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Key Product Details

Source

E. coli

Tag

N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

Applications

Antibody Competition
View all Adenine Nucleotide Translocator 2 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human Adenine Nucleotide Translocator 2.

Source: E. coli

Amino Acid Sequence: AAVSKTAVAPIERVKLLLQVQHASKQIS

Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

Purity

>80% by SDS-PAGE and Coomassie blue staining

Predicted Molecular Mass

21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Applications

Antibody Competition (10 - 100 molar excess)

Application Notes

This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP2-56762.

It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

Protein / Peptide Type

Recombinant Protein Antigen

Formulation, Preparation, and Storage

NBP2-56762PEP
Formulation PBS and 1M Urea, pH 7.4.
Preservative No Preservative
Concentration Please see the vial label for concentration. If unlisted please contact technical services.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20C. Avoid freeze-thaw cycles.

Background: Adenine Nucleotide Translocator 2

ADP/ATP translocase, the most abundant mitochondrial protein, is an integral component of the inner mitochondrial membrane. It facilitates exchange of ADP and ATP between the cytosol and the mitochondria, thereby linking the subcellular compartment of ATP production to those of ATP utilization. SLC25A5 is 1 of at least 3 transcriptionally active ADP/ATP translocase genes in humans (Chen et al., 1990 [PubMed 2157297]). Battini et al. (1987) [PubMed 3031073] cloned an ADP/ATP translocase gene from an Okayama-Berg library derived from SV40-transformed human fibroblasts. Ku et al. (1990) [PubMed 2168878] cloned and sequenced the ANT2 gene. Chen et al. (1990) [PubMed 2157297] isolated 7 ADP/ATP translocase pseudogenes from recombinant human genomic libraries. Each pseudogene sequence had more than 85% identity with the sequence of the translocase cDNA derived from fibroblast mRNA, but each had mutations that precluded synthesis of a functional protein.[supplied by OMIM]

Alternate Names

Adenine nucleotide translocator 2, adenine nucleotide translocator 2 (fibroblast), ADP, ADP/ATP carrier protein, ADP/ATP translocase 2, ANT2AAC2, 2F1, ATP carrier protein 2, ATP carrier protein, fibroblast isoform, solute carrier family 25 (mitochondrial carrier; adenine nucleotidetranslocator), member 5, Solute carrier family 25 member 5, T2, T3ANT 2

Gene Symbol

SLC25A5

Additional Adenine Nucleotide Translocator 2 Products

Product Documents for Adenine Nucleotide Translocator 2 Recombinant Protein Antigen

Certificate of Analysis

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Product Specific Notices for Adenine Nucleotide Translocator 2 Recombinant Protein Antigen

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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