Adenosine A2aR Antibody (3F6-9G5) - Humanized - Low Endotoxin, Azide and BSA Free

Novus Biologicals | Catalog # NBP3-28455

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Functional

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG1 Clone # 3F6-9G5 expressed in CHO

Format

Low Endotoxin, Azide and BSA Free
View all Adenosine A2aR Primary Antibodies »

Product Specifications

Immunogen

AA2AR / Adenosine A2aR

Clonality

Monoclonal

Host

Human

Isotype

IgG1

Description

The heavy chain type is IgG1, and the light chain type is hukappa. It has a predicted MW of 146.66 kDa.

This product is shipped at ambient temperature. Upon receipt, store immediately at -20C or lower for 12 months in a lyophilized state. - 80C for 3 months after reconstitution. Avoid repeated freeze-thaw cycles.

Endotoxin Level

< 0.001EU/ug,determined by LAL method.

Scientific Data Images

Adenosine A2aR Antibody (3F6-9G5) - Humanized

Flow Cytometry: Adenosine A2aR Antibody (3F6-9G5) - Humanized[NBP3-28455] -

THP-1 were stained with Adenosine A2aR Antibody (3F6-9G5) - Humanized and negative control protein respectively, washed and then followed by PE and analyzed with FACS,The EC50 was approximately 0.2161 ug/mL.
Adenosine A2aR Antibody (3F6-9G5) - Humanized

Flow Cytometry: Adenosine A2aR Antibody (3F6-9G5) - Humanized[NBP3-28455] -

Human A2AR HEK293 Cell Line were stained with Adenosine A2aR Antibody (3F6-9G5) - Humanized and negative control protein respectively, washed and then followed by PE and analyzed with FACS
Adenosine A2aR Antibody (3F6-9G5) - Humanized

Flow Cytometry: Adenosine A2aR Antibody (3F6-9G5) - Humanized[NBP3-28455] -

Cynomolgus / Rhesus Macaque A2AR HEK293 Cell Line were stained with Adenosine A2aR Antibody (3F6-9G5) - Humanized and negative control protein respectively, washed and then followed by PE and analyzed with FACS,The EC50 was approximately 0.215 ug/mL.

Applications

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Functional

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Reconstitute with sterile, distilled water to a final concentration of 1 mg/ml. Gently shake to solubilize completely. Do not vortex.

Formulation

Lyophilized from 25mM histidine, 8% sucrose, 0.01% Tween80 (pH6.2)

Format

Low Endotoxin, Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C in powder form. Store at -80C once reconstituted.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Adenosine A2aR

Adenosine A2a Receptor encodes a protein which is one of several receptor subtypes for adenosine. The activity of the encoded protein, a G-protein coupled receptor family member, is mediated by G proteins which activate adenylyl cyclase. The encoded protein is abundant in basal ganglia, vasculature and platelets and it is a major target of caffeine.

Long Name

Adenosine A2A Receptor

Alternate Names

A2aR, Adenosine A2a R, ADORA2A, RDC8

Gene Symbol

ADORA2A

UniProt

P29274

Additional Adenosine A2aR Products

Product Documents

Certificate of Analysis

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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